Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation
Joint Authors
Dong, Guoyi
Wang, Shengpeng
Ge, Yuping
Deng, Qiuting
Cao, Qi
Wang, Quanlei
Shang, Zhouchun
OuYang, Wenjie
Li, Jing
Liu, Chao
Tang, Jie
Zhao, Weihua
Gu, Ying
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-15
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Human mesenchymal stem cells (hMSCs) are widely used in clinical research because of their multipotential, immunomodulatory, and reparative properties.
Previous studies determined that hMSC spheroids from a three-dimensional (3D) culture possess higher therapeutic efficacy than conventional hMSCs from a monolayer (2D) culture.
To date, various 3D culture methods have been developed to form hMSC spheroids but most of them used culture medium containing fetal bovine serum (FBS), which is not suitable for further clinical use.
Here, we demonstrate that dissociated single MSCs seeded in induced pluripotent stem medium (MiPS) adhere loosely to the dish and spontaneously migrate to form spheroids during day 3 to day 6.
Through component deletion screening and complementation experiments, the knockout serum replacement (KSR) was identified as necessary and sufficient for hMSC spheroid formation.
Transcriptome analysis showed that the overall expression profiles were highly similar between 2D culture with FBS and KSR-derived spheroids.
Interestingly, genes related to inflammatory response, immune response, and angiogenesis were upregulated in spheroids at day 6 and qPCR results further validated the increased expression level of related genes, including STC1, CCL7, HGF, IL24, and TGFB3.
When spheroids were replated in normal FBS medium, cells formed a typical spindle-shaped morphology and FACS results showed that the recovered cells retained MSC-specific surface markers, such as CD73, CD90, and CD105.
In summary, we developed a practical and convenient method to generate hMSC spheroids for clinical research and therapy.
American Psychological Association (APA)
Dong, Guoyi& Wang, Shengpeng& Ge, Yuping& Deng, Qiuting& Cao, Qi& Wang, Quanlei…[et al.]. 2019. Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation. Stem Cells International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1209232
Modern Language Association (MLA)
Dong, Guoyi…[et al.]. Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation. Stem Cells International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1209232
American Medical Association (AMA)
Dong, Guoyi& Wang, Shengpeng& Ge, Yuping& Deng, Qiuting& Cao, Qi& Wang, Quanlei…[et al.]. Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1209232
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209232