Combining ECM Hydrogels of Cardiac Bioactivity with Stem Cells of High Cardiomyogenic Potential for Myocardial Repair
Joint Authors
Bai, Rui
Tian, Lei
Li, Yi
Zhang, Jiao
Wei, Yujie
Jin, Zhigeng
Liu, Zhiqiang
Liu, Huiliang
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-23
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Tissue engineering exploring the combination of scaffolds and seeding cells was proposed as a promising strategy for myocardial repair.
However, the therapeutic outcomes varied greatly due to different selection of scaffolds and seeding cells.
Herein, the potential of combining bioactive extracellular matrix (ECM) hydrogels and high cardiomyogenic seeding cells was explored for myocardial repair in vitro and in vivo.
Temperature-sensitive ECM hydrogels were prepared from decellularized rat hearts, and cardiomyogenic seeding cells were isolated from brown adipose (brown adipose-derived stem cells (BADSCs)).
The in vitro studies demonstrated that ECM hydrogel significantly supported the proliferation and cardiomyogenic differentiation of BADSCs.
Importantly, the function and maturation of BADSC-derived cardiomyocytes were also promoted as evidenced by Ca2+ transient’s measurement and protein marker expression.
After myocardial transplantation, the combination of BADSCs and ECM hydrogels significantly preserved cardiac function and chamber geometry compared with BADSCs or ECM hydrogels alone.
Meanwhile, the ECM hydrogel also enhanced BADSC engraftment and myocardial regeneration in vivo.
These results indicated that heart-derived ECM hydrogels exerted significant influence on the fate of cardiomyogenic cells toward benefiting myocardial repair, which may explain the enhanced stem cell therapy by the scaffold.
Collectively, it indicated that the combination of ECM hydrogel and the cardiomyogenic cells may represent a promising strategy for cardiac tissue engineering.
American Psychological Association (APA)
Bai, Rui& Tian, Lei& Li, Yi& Zhang, Jiao& Wei, Yujie& Jin, Zhigeng…[et al.]. 2019. Combining ECM Hydrogels of Cardiac Bioactivity with Stem Cells of High Cardiomyogenic Potential for Myocardial Repair. Stem Cells International،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1209306
Modern Language Association (MLA)
Bai, Rui…[et al.]. Combining ECM Hydrogels of Cardiac Bioactivity with Stem Cells of High Cardiomyogenic Potential for Myocardial Repair. Stem Cells International No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1209306
American Medical Association (AMA)
Bai, Rui& Tian, Lei& Li, Yi& Zhang, Jiao& Wei, Yujie& Jin, Zhigeng…[et al.]. Combining ECM Hydrogels of Cardiac Bioactivity with Stem Cells of High Cardiomyogenic Potential for Myocardial Repair. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1209306
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209306