rBMSCCav-1F92A Mediates Oxidative Stress in PAH Rat by Regulating SelW14-3-3η and CA1Kininogen Signal Transduction
Joint Authors
Zou, Cheng-wei
Yu, Wan-cheng
Chen, Hai-ying
Yang, Hong-li
Xia, Peng
Sun, Tong-wen
Wang, Le-xin
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-28
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Background/Objectives.
Carbonic anhydrase 1 (CA1)/kininogen and selenoprotein W (SelW)/14-3-3η signal transduction orchestrate oxidative stress, which can also be regulated by nitric oxide (NO).
The mutated caveolin-1 (Cav-1F92A) gene may enhance NO production.
This study explored the effect of Cav-1F92A-modified rat bone marrow mesenchymal stem cells (rBMSC/Cav-1F92A) on oxidative stress regulation through CA1/kininogen and SelW/14-3-3η signal transduction in a rat model of monocrotaline- (MCT-) induced pulmonary arterial hypertension (PAH).
Method.
PAH was induced in rats through the subcutaneous injection of MCT.
Next, rBMSC/Vector (negative control), rBMSC/Cav-1, rBMSC/Cav-1F92A, or rBMSC/Cav-1F92A+L-NAME were administered to the rats.
Changes in pulmonary hemodynamic and vascular morphometry and oxidative stress levels were evaluated.
CA1/kininogen and SelW/14-3-3η signal transduction, endothelial nitric oxide synthase (eNOS) dimerization, and eNOS/NO/sGC/cGMP pathway changes were determined through real-time polymerase chain reaction, Western blot, or immunohistochemical analyses.
Results.
In MCT-induced PAH rats, rBMSC/Cav-1F92A treatment reduced right ventricular systolic pressure, vascular stenosis, and oxidative stress; downregulated CA1/kininogen signal transduction; upregulated SelW/14-3-3η signal transduction; and reactivated the NO pathway.
Conclusions.
In a rat model of MCT-induced PAH, rBMSC/Cav-1F92A reduced oxidative stress by regulating CA1/kininogen and SelW/14-3-3η signal transduction.
American Psychological Association (APA)
Yu, Wan-cheng& Chen, Hai-ying& Yang, Hong-li& Xia, Peng& Zou, Cheng-wei& Sun, Tong-wen…[et al.]. 2019. rBMSCCav-1F92A Mediates Oxidative Stress in PAH Rat by Regulating SelW14-3-3η and CA1Kininogen Signal Transduction. Stem Cells International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1209308
Modern Language Association (MLA)
Yu, Wan-cheng…[et al.]. rBMSCCav-1F92A Mediates Oxidative Stress in PAH Rat by Regulating SelW14-3-3η and CA1Kininogen Signal Transduction. Stem Cells International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1209308
American Medical Association (AMA)
Yu, Wan-cheng& Chen, Hai-ying& Yang, Hong-li& Xia, Peng& Zou, Cheng-wei& Sun, Tong-wen…[et al.]. rBMSCCav-1F92A Mediates Oxidative Stress in PAH Rat by Regulating SelW14-3-3η and CA1Kininogen Signal Transduction. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1209308
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209308