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Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells
Joint Authors
Rovai, E. S.
Holzhausen, M.
Sipert, Carla
Ambrósio, Lucas Macedo Batitucci
de França, Bruno Nunes
de Oliveira, Letícia Rodrigues
Gasparoni, Letícia Miquelitto
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-02
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Protease-activated receptor 1 (PAR1) has been associated to tissue repair and bone healing.
The aim of the present study was to evaluate the effect of PAR1 activation on the osteogenic activity of human periodontal ligament stem cells (PDLSCs).
PDLSCs were cultured in the presence of PAR1-selective agonist peptide (100 nM), thrombin (0.1 U/mL), or PAR1 antagonist peptide (100 nM).
Calcium deposits, calcium concentration (supernatant), alkaline phosphatase activity (ALP), cell proliferation, and gene (qPCR) and protein expression (ELISA assay) of osteogenic factors were assessed at 2, 7, and 14 days.
PAR1 activation led to increased calcium deposits (p<0.05), calcium concentration (p<0.05), ALP activity (p<0.05), and cell proliferation (p<0.05).
Further, PAR1 activation may increase gene and protein expression of Runx2 (p<0.05) and OPG (p<0.05).
In conclusion, PAR1 activation increases osteogenic activity of PDLSCs, providing a possible new strategy for periodontal regenerative therapies.
American Psychological Association (APA)
Rovai, E. S.& Ambrósio, Lucas Macedo Batitucci& de França, Bruno Nunes& de Oliveira, Letícia Rodrigues& Gasparoni, Letícia Miquelitto& Sipert, Carla…[et al.]. 2019. Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1209352
Modern Language Association (MLA)
Rovai, E. S.…[et al.]. Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells. Stem Cells International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1209352
American Medical Association (AMA)
Rovai, E. S.& Ambrósio, Lucas Macedo Batitucci& de França, Bruno Nunes& de Oliveira, Letícia Rodrigues& Gasparoni, Letícia Miquelitto& Sipert, Carla…[et al.]. Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1209352
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209352