![](/images/graphics-bg.png)
Pretreatment with AM1241 Enhances the Analgesic Effect of Intrathecally Administrated Mesenchymal Stem Cells
Joint Authors
Xie, Junran
Ren, Jinxuan
Liu, Na
Wu, Chengwei
Xiao, Dongju
Luo, Huanyu
Du, Jingxian
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-10
Country of Publication
Egypt
No. of Pages
17
Abstract EN
Mesenchymal stem cells have cannabinoid (CB) receptors type 1 and type 2 and can alleviate a variety of neuropathic pains, including chronic constriction injury (CCI).
A selective CB2 receptor agonist is AM1241.
In the present study, it was found that mice with CCI displayed a longer duration of mechanical and thermal analgesia when intrathecally (i.t.) injected with AM1241-treated mesenchymal stem cells, compared to those injected with untreated mesenchymal stem cells or AM1241 alone.
Moreover, CCI-induced upregulation of the phosphorylated extracellular signal-regulated kinase (ERK) 1/2 (p-ERK1/2) was inhibited following i.t.
injection of AM1241-treated mesenchymal stem cells and this inhibition was noticeably higher compared to injection with untreated mesenchymal stem cells.
The expression of transforming growth factor-β1 (TGF-β1) was also analyzed in the dorsal root ganglion (DRGs) and spinal cord of CCI mice.
In untreated CCI mice, expression of TGF-β1 was increased, whereas pretreatment with AM1241-treated mesenchymal stem cells regulated the expression of TGF-β1 on 10 days and 19 days after surgery.
In addition, i.t.
injection of exogenous TGF-β1 slightly alleviated neuropathic pain whilst neutralization of TGF-β1 potently blocked the effect of AM1241-treated mesenchymal stem cells on thermal hyperalgesia and mechanical allodynia of CCI mice.
In an in vitro experiment, AM1241 could enhance the release of TGF-β1 in the supernatant of BMSCs after lipopolysaccharide (LPS) simulation.
Taken together, the findings of the current study show that i.t.
administration of AM1241-treated mesenchymal stem cells has a positive effect on analgesia and that TGF-β1 and p-ERK1/2 may be the molecular signaling pathway involved in this process.
American Psychological Association (APA)
Xie, Junran& Ren, Jinxuan& Liu, Na& Wu, Chengwei& Xiao, Dongju& Luo, Huanyu…[et al.]. 2019. Pretreatment with AM1241 Enhances the Analgesic Effect of Intrathecally Administrated Mesenchymal Stem Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1209421
Modern Language Association (MLA)
Xie, Junran…[et al.]. Pretreatment with AM1241 Enhances the Analgesic Effect of Intrathecally Administrated Mesenchymal Stem Cells. Stem Cells International No. 2019 (2019), pp.1-17.
https://search.emarefa.net/detail/BIM-1209421
American Medical Association (AMA)
Xie, Junran& Ren, Jinxuan& Liu, Na& Wu, Chengwei& Xiao, Dongju& Luo, Huanyu…[et al.]. Pretreatment with AM1241 Enhances the Analgesic Effect of Intrathecally Administrated Mesenchymal Stem Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1209421
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209421