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Influence of Lineage-Negative Stem Cell Therapy on Articulatory Functions in ALS Patients
Joint Authors
Machalinski, Boguslaw
Paczkowska, Edyta
Gołąb-Janowska, Monika
Meller, Agnieszka
Rotter, Iwona
Łuczkowska, Karolina
Nowacki, Przemysław
Pawlukowska, Wioletta
Baumert, Bartłomiej
Sobuś, Anna
Wełnicka, Agnieszka
Machowska-Sempruch, Karolina
Zawiślak, Alicja
Milczarek, Sławomir
Osękowska, Bogumiła
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-02
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Introduction.
Amyotrophic lateral sclerosis (ALS) is a fatal, neurodegenerative disease, leading to loss of muscle strength and motor control.
Impaired speech and swallowing lower the quality of life and consequently may induce acute respiratory failure.
Bone marrow-derived stem and progenitor cells (SPCs) may be a valuable source of trophic factors.
In this study, we assessed whether adjuvant cellular therapy could affect the levels of selected neurotrophins and proinflammatory factors in the cerebrospinal fluid (CSF) and subsequently prevent the deterioration of articulation.
Materials and Methods.
The study group consisted of 32 patients with sporadic ALS who underwent autologous lineage-negative (Lin−) stem cell intrathecal administration to the spinal canal.
Lin− cells were aspirated from the bone marrow and isolated using immunomagnetic beads and a lineage cell depletion kit.
Patients were examined for articulatory functions by means of the Voice Handicap Index (VHI) questionnaire and Frenchay Dysarthria Assessment (FDA).
In parallel, we carried out the analysis of selected trophic and proinflammatory factors in CSF utilizing multiplex fluorescent bead-based immunoassays.
Results.
Of the 32 patients who received the Lin− progenitor cell therapy, 6 (group I) showed improvement in articulatory functions, 23 remained stable (group II), and 3 deteriorated (group III) on the 28th day.
The improvement was particularly noticeable in a better cough reflex, laryngeal time, and dribble reflex.
A statistically significant lower level of brain-derived neurotrophic factor (BDNF) was observed on day 0 in group I compared to group II.
The CSF concentrations of C-reactive protein (CRP) in group I significantly decreased 7 days after Lin− SPC transplantation.
On the contrary, a significant increase in the tumor necrosis factor receptor (TNF-R) level was confirmed among patients from group I with improvement of dribble and coughing reflex, tongue movements, and respiration on the 7th day, as well as on day 28 including dribble reflex solely.
Conclusions.
An application of Lin− stem cells could potentate the beneficial humoral effect.
The prevention of deterioration of articulatory functions in ALS patients after applying adjuvant Lin− stem cell therapy seems to be promising.
Although the procedure is safe and feasible, it requires further in-depth studies.
American Psychological Association (APA)
Pawlukowska, Wioletta& Baumert, Bartłomiej& Gołąb-Janowska, Monika& Sobuś, Anna& Wełnicka, Agnieszka& Meller, Agnieszka…[et al.]. 2019. Influence of Lineage-Negative Stem Cell Therapy on Articulatory Functions in ALS Patients. Stem Cells International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1209498
Modern Language Association (MLA)
Pawlukowska, Wioletta…[et al.]. Influence of Lineage-Negative Stem Cell Therapy on Articulatory Functions in ALS Patients. Stem Cells International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1209498
American Medical Association (AMA)
Pawlukowska, Wioletta& Baumert, Bartłomiej& Gołąb-Janowska, Monika& Sobuś, Anna& Wełnicka, Agnieszka& Meller, Agnieszka…[et al.]. Influence of Lineage-Negative Stem Cell Therapy on Articulatory Functions in ALS Patients. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1209498
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209498