α2-HS Glycoprotein in Plasma Extracellular Vesicles Inhibits the Osteogenic Differentiation of Human Mesenchymal Stromal Cells In Vitro
Joint Authors
Wang, Peng
Xie, Zhongyu
Wu, Yanfeng
Su, Hongjun
Li, Jinteng
Deng, Wen
Wu, Xiaohua
Wang, Shan
Ma, Mengjun
Feng, Pei
Su, Chunyan
Yang, Jiewen
Tang, Su’an
Shen, Huiyong
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-07
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Extracellular vesicles (Evs) contain diverse functional proteins, mRNAs, miRNAs, and DNA fragments, are secreted by various types of cells, and play important roles in cellular communication.
Here, we show for the first time that plasma Evs inhibited the osteogenic differentiation of mesenchymal stromal cells (MSCs) in vitro and the level of inhibition was positively correlated with the plasma Evs concentration.
Plasma Evs downregulated the expression of markers such as osteocalcin (OCN), Runt-related transcription factor 2 (Runx2), and Osterix at mRNA levels required for osteogenic differentiation and reduced pSmad1/5/8 levels in MSCs.
Furthermore, pSmad1/5/8 levels increased and MSCs underwent normal osteogenic differentiation after Evs-derived α2-HS glycoprotein (AHSG) function was inhibited with an anti-AHSG neutralizing antibody.
However, the levels of pERK1/2, active β-catenin, and HES1 were not significantly altered.
Therefore, we propose that as essential components of the extracellular microenvironment of MSCs, plasma Evs are taken up by MSCs and subsequently repress osteogenic differentiation through an AHSG-mediated decrease in pSmad1/5/8 levels.
Our work identifies plasma Evs as novel regulators of MSC osteogenic differentiation.
American Psychological Association (APA)
Wu, Xiaohua& Ma, Mengjun& Wang, Peng& Xie, Zhongyu& Wang, Shan& Su, Hongjun…[et al.]. 2019. α2-HS Glycoprotein in Plasma Extracellular Vesicles Inhibits the Osteogenic Differentiation of Human Mesenchymal Stromal Cells In Vitro. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1209509
Modern Language Association (MLA)
Wu, Xiaohua…[et al.]. α2-HS Glycoprotein in Plasma Extracellular Vesicles Inhibits the Osteogenic Differentiation of Human Mesenchymal Stromal Cells In Vitro. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1209509
American Medical Association (AMA)
Wu, Xiaohua& Ma, Mengjun& Wang, Peng& Xie, Zhongyu& Wang, Shan& Su, Hongjun…[et al.]. α2-HS Glycoprotein in Plasma Extracellular Vesicles Inhibits the Osteogenic Differentiation of Human Mesenchymal Stromal Cells In Vitro. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1209509
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209509