Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells
Joint Authors
Liu, Guanqi
Lu, Yeming
Mai, Zhihui
Liu, Runheng
Peng, Zhuli
Chen, Lin
Chen, Zheng
Wang, Ruizhi
Ai, Hong
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-04
Country of Publication
Egypt
No. of Pages
13
Abstract EN
MicroRNAs (miRNAs) have been widely demonstrated to interact with multiple cellular signaling pathways and to participate in a wide range of physiological processes.
Estradiol-17β (E2) is the most potent and prevalent endogenous estrogen that plays a vital role in promoting bone formation and reducing bone resorption.
Currently, little is known about the regulation of miRNAs in E2-induced osteogenic differentiation.
In the present study, the primary bone marrow mesenchymal stem cells from rats (rBMSCs) were isolated and incubated with E2, followed by miRNA profiling.
The microarray showed that 29 miRNAs were differentially expressed in response to E2 stimulation.
Further verification by real-time reverse-transcriptase polymerase chain reaction revealed that E2 enhanced the expression of let-7b and miR-25 but suppressed the miR-30b expression.
Moreover, a gain-of-function experiment confirmed that miR-30b negatively regulated the E2-induced osteogenic differentiation.
These data suggest an important role of miRNAs in osteogenic differentiation.
American Psychological Association (APA)
Liu, Guanqi& Lu, Yeming& Mai, Zhihui& Liu, Runheng& Peng, Zhuli& Chen, Lin…[et al.]. 2019. Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1209565
Modern Language Association (MLA)
Liu, Guanqi…[et al.]. Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1209565
American Medical Association (AMA)
Liu, Guanqi& Lu, Yeming& Mai, Zhihui& Liu, Runheng& Peng, Zhuli& Chen, Lin…[et al.]. Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1209565
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209565