A Three-Dimensional Culture Model of Reversibly Quiescent Myogenic Cells
Joint Authors
Naro, Fabio
Pellegrini, Manuela
Aguanno, Salvatore
Petrelli, Claudia
Di Siena, Sara
De Angelis, Luciana
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-11
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Satellite cells (SC) are the stem cells of skeletal muscles.
They are quiescent in adult animals but resume proliferation to allow muscle hypertrophy or regeneration after injury.
The mechanisms balancing quiescence, self-renewal, and differentiation of SC are difficult to analyze in vivo owing to their complexity and in vitro because the staminal character of SC is lost when they are removed from the niche and is not adequately reproduced in the culture models currently available.
To overcome these difficulties, we set up a culture model of the myogenic C2C12 cell line in suspension.
When C2C12 cells are cultured in suspension, they enter a state of quiescence and form three-dimensional aggregates (myospheres) that produce the extracellular matrix and express markers of quiescent SC.
In the initial phase of culture, a portion of the cells fuses in syncytia and abandons the myospheres.
The remaining cells are mononucleated and quiescent but resume proliferation and differentiation when plated in a monolayer.
The notch pathway controls the quiescent state of the cells as shown by the fact that its inhibition leads to the resumption of differentiation.
Within this context, notch3 appears to play a central role in the activity of this pathway since the expression of notch1 declines soon after aggregation.
In summary, the culture model of C2C12 in suspension may be used to study the cellular interactions of muscle stem cells and the pathways controlling SC quiescence entrance and maintenance.
American Psychological Association (APA)
Aguanno, Salvatore& Petrelli, Claudia& Di Siena, Sara& De Angelis, Luciana& Pellegrini, Manuela& Naro, Fabio. 2019. A Three-Dimensional Culture Model of Reversibly Quiescent Myogenic Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1209570
Modern Language Association (MLA)
Aguanno, Salvatore…[et al.]. A Three-Dimensional Culture Model of Reversibly Quiescent Myogenic Cells. Stem Cells International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1209570
American Medical Association (AMA)
Aguanno, Salvatore& Petrelli, Claudia& Di Siena, Sara& De Angelis, Luciana& Pellegrini, Manuela& Naro, Fabio. A Three-Dimensional Culture Model of Reversibly Quiescent Myogenic Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1209570
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209570