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Neuronal Transdifferentiation Potential of Human Mesenchymal Stem Cells from Neonatal and Adult Sources by a Small Molecule Cocktail
Joint Authors
Álvarez-Pérez, Marco Antonio
Montesinos, Juan J.
Hernández-Estévez, E.
Cortés-Medina, Lorena V.
Pasantes-Morales, Herminia
Aguilera-Castrejon, Alejandro
Picones, Arturo
Lara-Figueroa, Cesar O.
Luis, Enoch
Cortés-Morales, Victor A.
De la Rosa Ruiz, M. P.
Ramos-Mandujano, Gerardo
Bonifaz, Laura
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-01
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Human mesenchymal stem cells (MSCs) are good candidates for brain cell replacement strategies and have already been used as adjuvant treatments in neurological disorders.
MSCs can be obtained from many different sources, and the present study compares the potential of neuronal transdifferentiation in MSCs from adult and neonatal sources (Wharton’s jelly (WhJ), dental pulp (DP), periodontal ligament (PDL), gingival tissue (GT), dermis (SK), placenta (PLAC), and umbilical cord blood (UCB)) with a protocol previously tested in bone marrow- (BM-) MSCs consisting of a cocktail of six small molecules: I-BET151, CHIR99021, forskolin, RepSox, Y-27632, and dbcAMP (ICFRYA).
Neuronal morphology and the presence of cells positive for neuronal markers (TUJ1 and MAP2) were considered attributes of neuronal induction.
The ICFRYA cocktail did not induce neuronal features in WhJ-MSCs, and these features were only partial in the MSCs from dental tissues, SK-MSCs, and PLAC-MSCs.
The best response was found in UCB-MSCs, which was comparable to the response of BM-MSCs.
The addition of neurotrophic factors to the ICFRYA cocktail significantly increased the number of cells with complex neuron-like morphology and increased the number of cells positive for mature neuronal markers in BM- and UCB-MSCs.
The neuronal cells generated from UCB-MSCs and BM-MSCs showed increased reactivity of the neuronal genes TUJ1, MAP2, NF-H, NCAM, ND1, TAU, ENO2, GABA, and NeuN as well as down- and upregulation of MSC and neuronal genes, respectively.
The present study showed marked differences between the MSCs from different sources in response to the transdifferentiation protocol used here.
These results may contribute to identifying the best source of MSCs for potential cell replacement therapies.
American Psychological Association (APA)
Cortés-Medina, Lorena V.& Pasantes-Morales, Herminia& Aguilera-Castrejon, Alejandro& Picones, Arturo& Lara-Figueroa, Cesar O.& Luis, Enoch…[et al.]. 2019. Neuronal Transdifferentiation Potential of Human Mesenchymal Stem Cells from Neonatal and Adult Sources by a Small Molecule Cocktail. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1209575
Modern Language Association (MLA)
Cortés-Medina, Lorena V.…[et al.]. Neuronal Transdifferentiation Potential of Human Mesenchymal Stem Cells from Neonatal and Adult Sources by a Small Molecule Cocktail. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1209575
American Medical Association (AMA)
Cortés-Medina, Lorena V.& Pasantes-Morales, Herminia& Aguilera-Castrejon, Alejandro& Picones, Arturo& Lara-Figueroa, Cesar O.& Luis, Enoch…[et al.]. Neuronal Transdifferentiation Potential of Human Mesenchymal Stem Cells from Neonatal and Adult Sources by a Small Molecule Cocktail. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1209575
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209575