Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA
Joint Authors
Schlensak, Christian
Avci-Adali, Meltem
Steinle, Heidrun
Weber, Marbod
Behring, Andreas
Mau-Holzmann, Ulrike
Wendel, Hans-Peter
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-19
Country of Publication
Egypt
No. of Pages
16
Abstract EN
The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is gaining in importance in the fields of regenerative medicine, tissue engineering, and disease modeling.
Patient-specific iPSCs have as an unlimited cell source a tremendous potential for generating various types of autologous cells.
For the future clinical applicability of these iPSC-derived cells, the generation of iPSCs via nongenome integrating methods and the efficient reprogramming of patients’ somatic cells are required.
In this study, 2 different RNA-based footprint-free methods for the generation of iPSCs were compared: the use of synthetic modified messenger RNAs (mRNAs) or self-replicating RNAs (srRNAs) encoding the reprogramming factors and GFP.
Using both RNA-based methods, integration-free iPSCs without genomic alterations were obtained.
The pluripotency characteristics identified by specific marker detection and the in vitro and in vivo trilineage differentiation capacity were comparable.
Moreover, the incorporation of a GFP encoding sequence into the srRNA enabled a direct and convenient monitoring of the reprogramming procedure and the successful detection of srRNA translation in the transfected cells.
Nevertheless, the use of a single srRNA to induce pluripotency was less time consuming, faster, and more efficient than the daily transfection of cells with synthetic mRNAs.
Therefore, we believe that the srRNA-based approach might be more appropriate and efficient for the reprogramming of different types of somatic cells for clinical applications.
American Psychological Association (APA)
Steinle, Heidrun& Weber, Marbod& Behring, Andreas& Mau-Holzmann, Ulrike& Schlensak, Christian& Wendel, Hans-Peter…[et al.]. 2019. Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA. Stem Cells International،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1209578
Modern Language Association (MLA)
Steinle, Heidrun…[et al.]. Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA. Stem Cells International No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1209578
American Medical Association (AMA)
Steinle, Heidrun& Weber, Marbod& Behring, Andreas& Mau-Holzmann, Ulrike& Schlensak, Christian& Wendel, Hans-Peter…[et al.]. Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1209578
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209578