Genes Involved in the Transcriptional Regulation of Pluripotency Are Expressed in Malignant Tumors of the Uterine Cervix and Can Induce Tumorigenic Capacity in a Nontumorigenic Cell Line
Joint Authors
Gariglio, Patricio
Pérez-Plasencia, Carlos
Ocadiz-Delgado, Rodolfo
Valencia-González, Heriberto Abraham
Ruíz, Graciela
García-Carrancá, Alejandro
Pérez-Montiel, Delia
Muñoz, Felipe
Fernández-Retana, Jorge
Reséndis-Antonio, Osbaldo
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-01
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Transcription factors OCT4, SOX2, KLF4, C-MYC, and NANOG (OSKM-N) regulate pluripotency and stemness, and their ectopic expression reprograms human and murine fibroblasts that constitute the key of regenerative medicine.
To determine their contribution to cell transformation, we analyzed the gene expression profiles of these transcription factors in cervical cancer samples and found that they are preferentially expressed in the tumor component.
Also, cancer stem cell-enriched cultures grown as sphere cultures showed overexpression of OSKM-N genes.
Importantly, we observed that lentiviral-mediated transduction of these factors confers, to a nontumorigenic immortalized human cell line, properties of cancer stem cells as the ability to form tumors in a mouse model.
When we performed a meta-analysis using microarray data from cervical cancer biopsies and normal tissues, we found that the expression of OSKM-N and some target genes allowed separating tumor and normal tissues between samples, which enhanced the importance of OSKM-N in the tumorigenesis.
Finally, we analyzed and compared both transcript and protein expression profiles of these factors within a cohort of patients with cervical cancer.
To our knowledge, this is the first time that the expression of OSKM-N is described to induce one of the main characteristics of the cancer stem cell, the tumorigenicity.
And, more importantly, its exogenous expression in a nontumorigenic cell line is sufficient to induce a tumorigenic phenotype; furthermore, the differential expression of this transcription factor distinguishes tumor tissue and normal tissue in cervical samples.
American Psychological Association (APA)
Ruíz, Graciela& Valencia-González, Heriberto Abraham& Pérez-Montiel, Delia& Muñoz, Felipe& Ocadiz-Delgado, Rodolfo& Fernández-Retana, Jorge…[et al.]. 2019. Genes Involved in the Transcriptional Regulation of Pluripotency Are Expressed in Malignant Tumors of the Uterine Cervix and Can Induce Tumorigenic Capacity in a Nontumorigenic Cell Line. Stem Cells International،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1209589
Modern Language Association (MLA)
Ruíz, Graciela…[et al.]. Genes Involved in the Transcriptional Regulation of Pluripotency Are Expressed in Malignant Tumors of the Uterine Cervix and Can Induce Tumorigenic Capacity in a Nontumorigenic Cell Line. Stem Cells International No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1209589
American Medical Association (AMA)
Ruíz, Graciela& Valencia-González, Heriberto Abraham& Pérez-Montiel, Delia& Muñoz, Felipe& Ocadiz-Delgado, Rodolfo& Fernández-Retana, Jorge…[et al.]. Genes Involved in the Transcriptional Regulation of Pluripotency Are Expressed in Malignant Tumors of the Uterine Cervix and Can Induce Tumorigenic Capacity in a Nontumorigenic Cell Line. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1209589
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209589