Spontaneously Formed Spheroids from Mouse Compact Bone-Derived Cells Retain Highly Potent Stem Cells with Enhanced Differentiation Capability
Joint Authors
Kagami, Hideaki
Chen, Kai
Li, Xianqi
Li, Ni
Dong, Hongwei
Zhang, Yiming
Yoshizawa, Michiko
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-05
Country of Publication
Egypt
No. of Pages
13
Abstract EN
The results from our recent study showed the presence of two distinct spheroid-forming mechanisms, i.e., spontaneous and mechanical.
In this study, we focused on the spontaneously formed spheroids, and the character of spontaneously formed spheroids from mouse compact bone-derived cells (CBDCs) was explored.
Cells from (C57BL/6J) mouse leg bones were isolated, and compact bone-derived cells were cultured after enzymatic digestion.
Spontaneous spheroid formation was achieved on a culture plate with specific water contact angle as reported.
The expression levels of embryonic stem cell markers were analyzed using immunofluorescence and quantitative reverse transcription polymerase chain reaction.
Then, the cells from spheroids were induced into osteogenic and neurogenic lineages.
The spontaneously formed spheroids from CBDCs were positive for ES cell markers such as SSEA1, Sox2, Oct4, and Nanog.
Additionally, the expressions of fucosyltransferase 4/FUT4 (SSEA1), Sox2, and Nanog were significantly higher than those in monolayer cultured cells.
The gene expression of mesenchymal stem cell markers was almost identical in both spheroids and monolayer-cultured cells, but the expression of Sca-1 was higher in spheroids.
Spheroid-derived cells showed significantly higher osteogenic and neurogenic marker expression than monolayer-cultured cells after induction.
Spontaneously formed spheroids expressed stem cell markers and showed enhanced osteogenic and neurogenic differentiation capabilities than cells from the conventional monolayer culture, which supports the superior stemness.
American Psychological Association (APA)
Chen, Kai& Li, Xianqi& Li, Ni& Dong, Hongwei& Zhang, Yiming& Kagami, Hideaki…[et al.]. 2019. Spontaneously Formed Spheroids from Mouse Compact Bone-Derived Cells Retain Highly Potent Stem Cells with Enhanced Differentiation Capability. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1209743
Modern Language Association (MLA)
Kagami, Hideaki…[et al.]. Spontaneously Formed Spheroids from Mouse Compact Bone-Derived Cells Retain Highly Potent Stem Cells with Enhanced Differentiation Capability. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1209743
American Medical Association (AMA)
Chen, Kai& Li, Xianqi& Li, Ni& Dong, Hongwei& Zhang, Yiming& Kagami, Hideaki…[et al.]. Spontaneously Formed Spheroids from Mouse Compact Bone-Derived Cells Retain Highly Potent Stem Cells with Enhanced Differentiation Capability. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1209743
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209743