Designing Microfluidic Devices to Sort Haematopoietic Stem Cells Based on Their Mechanical Properties
Joint Authors
Du, Mingming
Kavanagh, Dean
Kalia, Neena
Zhang, Z.
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-05
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Aim.
Few haematopoietic stem cells (HSCs) injected systemically for therapeutic purposes actually reach sites of injury as the vast majority become entrapped within pulmonary capillaries.
One promising approach to maintain circulating HSC numbers would be to separate subpopulations with smaller size and/or greater deformability from a heterogeneous population.
This study tested whether this could be achieved using label-free microfluidic devices.
Methods.
2 straight (A-B) and 3 spiral (C-E) devices were fabricated with different dimensions.
Cell sorting was performed at different flow rates after which cell diameter and stiffness were determined using micromanipulation.
Cells isolated using the most efficient device were tested intravitally for their ability to home to the mouse injured gut.
Results.
Only straight Device B at a high flow rate separated HSCs with different mechanical properties.
Side outlets collected mostly deformable cells (nominal rupture stress/σR=6.81 kPa; coefficient of variation/CV=0.31) at a throughput of 2.3×105 cells/min.
All spiral devices at high flow rates separated HSCs with different stiffness and size.
Inner outlets collected mostly deformable cells in Devices C (σR=25.06 kPa; CV=0.26), D (σR=22.21 kPa; CV=0.41), and E (σR=29.26 kPa; CV=0.27) at throughputs of 2.3×105 cells/min, 1.5×105 cells/min, and 1.6×105 cells/min, respectively.
Since Device C separated cells with higher efficiency and throughput, it was utilized to test the homing ability of separated cells in vivo.
Significantly more deformable cells were observed trafficking through the injured gut—interestingly, increased retention was not observed.
Conclusion.
This study applied microfluidics to separate subpopulations from one stem cell type based on their intrinsic mechanical heterogeneity.
Fluid dynamics within curved devices most effectively separated HSCs.
Such devices may benefit cellular therapy.
American Psychological Association (APA)
Du, Mingming& Kavanagh, Dean& Zhang, Z.& Kalia, Neena. 2019. Designing Microfluidic Devices to Sort Haematopoietic Stem Cells Based on Their Mechanical Properties. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1209789
Modern Language Association (MLA)
Du, Mingming…[et al.]. Designing Microfluidic Devices to Sort Haematopoietic Stem Cells Based on Their Mechanical Properties. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1209789
American Medical Association (AMA)
Du, Mingming& Kavanagh, Dean& Zhang, Z.& Kalia, Neena. Designing Microfluidic Devices to Sort Haematopoietic Stem Cells Based on Their Mechanical Properties. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1209789
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209789