Preconditioning of Human Dental Pulp Stem Cells with Leukocyte- and Platelet-Rich Fibrin-Derived Factors Does Not Enhance Their Neuroregenerative Effect
Joint Authors
Ratajczak, Jessica
Bronckaers, Annelies
Dillen, Yörg
Gervois, Pascal
Vangansewinkel, Tim
Wolfs, Esther
Merckx, Greet
Lambrichts, Ivo
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-08
Country of Publication
Egypt
No. of Pages
15
Abstract EN
Pathologies of the central nervous system are characterized by loss of brain tissue and neuronal function which cannot be adequately restored by endogenous repair processes.
This stresses the need for novel treatment options such as cell-based therapies that are able to restore damaged tissue or stimulate repair.
This study investigated the neuroregenerative potential of the conditioned medium of human dental pulp stem cells (CM-hDPSCs) on neural stem cell (NSC) proliferation and migration as well as on neurite outgrowth of primary cortical neurons (pCNs).
Additionally, the effect of leukocyte- and platelet-rich fibrin (L-PRF) priming on the neuroregenerative potential of the hDPSC secretome on NSCs and pCNs was evaluated.
L-PRF contains factors that enhance stem cell-induced regeneration, but its effect on hDPSC-mediated neuroregeneration is unknown.
This study demonstrated that CM-hDPSCs enhanced neuritogenesis.
Moreover, CM-hDPSCs had a chemoattractant effect on NSCs.
Although priming hDPSCs with L-PRF increased brain-derived neurotrophic factor secretion, no additional effects on the paracrine-mediated repair mechanisms were observed.
These data support the neuroregenerative potential of hDPSCs, and although priming had no additional effect, the potential of L-PRF-primed hDPSCs on distinct regenerative mechanisms remains to be clarified.
American Psychological Association (APA)
Gervois, Pascal& Ratajczak, Jessica& Wolfs, Esther& Vangansewinkel, Tim& Dillen, Yörg& Merckx, Greet…[et al.]. 2019. Preconditioning of Human Dental Pulp Stem Cells with Leukocyte- and Platelet-Rich Fibrin-Derived Factors Does Not Enhance Their Neuroregenerative Effect. Stem Cells International،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1209796
Modern Language Association (MLA)
Gervois, Pascal…[et al.]. Preconditioning of Human Dental Pulp Stem Cells with Leukocyte- and Platelet-Rich Fibrin-Derived Factors Does Not Enhance Their Neuroregenerative Effect. Stem Cells International No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1209796
American Medical Association (AMA)
Gervois, Pascal& Ratajczak, Jessica& Wolfs, Esther& Vangansewinkel, Tim& Dillen, Yörg& Merckx, Greet…[et al.]. Preconditioning of Human Dental Pulp Stem Cells with Leukocyte- and Platelet-Rich Fibrin-Derived Factors Does Not Enhance Their Neuroregenerative Effect. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1209796
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209796