Islet1 and Brn3 Expression Pattern Study in Human Retina and hiPSC-Derived Retinal Organoid
Joint Authors
Hu, Dongpeng
Ge, Jian
Xian, Bikun
Luo, Ziming
Li, Kaijing
Tang, Mingjun
Xu, Chaochao
Liu, Yuchun
Li, Kang
Liu, Ying
Rong, Huifeng
Yang, Sijing
Ye, Meifang
Zhong, Xiufeng
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-10
Country of Publication
Egypt
No. of Pages
14
Abstract EN
This study was conducted to determine the dynamic Islet1 and Brn3 (POU4F) expression pattern in the human fetal retina and human-induced pluripotent stem cell- (hiPSC-) derived retinal organoid.
Human fetal eyes from 8 to 27 fetal weeks (Fwks), human adult retina, hiPSC-derived retinal organoid from 7 to 31 differentiation weeks (Dwks), and rhesus adult retina were collected for cyrosectioning.
Immunofluorescence analysis showed that Islet1 was expressed in retinal ganglion cells in the fetal retina, human adult retina, and retinal organoids.
Unexpectedly, after Fwk 20, Brn3 expression gradually decreased in the fetal retina.
In the midstage of development, Islet1 was detected in bipolar and developing horizontal cells.
As the photoreceptor developed, the Islet1-positive cone precursors gradually became Islet1-negative/S-opsin-positive cones.
This study highlights the distinguishing characteristics of Islet1 dynamic expression in human fetal retina development and proposes more concerns which should be taken regarding Brn3 as a cell-identifying marker in mature primate retina.
American Psychological Association (APA)
Luo, Ziming& Xu, Chaochao& Li, Kaijing& Xian, Bikun& Liu, Yuchun& Li, Kang…[et al.]. 2019. Islet1 and Brn3 Expression Pattern Study in Human Retina and hiPSC-Derived Retinal Organoid. Stem Cells International،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1209841
Modern Language Association (MLA)
Luo, Ziming…[et al.]. Islet1 and Brn3 Expression Pattern Study in Human Retina and hiPSC-Derived Retinal Organoid. Stem Cells International No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1209841
American Medical Association (AMA)
Luo, Ziming& Xu, Chaochao& Li, Kaijing& Xian, Bikun& Liu, Yuchun& Li, Kang…[et al.]. Islet1 and Brn3 Expression Pattern Study in Human Retina and hiPSC-Derived Retinal Organoid. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1209841
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209841