Isolation, Characterization, and Safety Evaluation of Human Skin-Derived Precursors from an Adherent Monolayer Culture System
Joint Authors
Dai, Ru
Xiong, Lidan
Hua, Wei
Chen, Wei
Li, Li
Li, Yiming
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-22, 22 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-19
Country of Publication
Egypt
No. of Pages
22
Abstract EN
Background.
Skin-derived precursors (SKPs) are promising candidates for regenerative medicine.
Several studies have transcultured human SKPs (termed tSKPs) from fibroblasts (FBs) expanded in monolayer culture.
Herein, we optimized the procedure by treating flasks with poly-2-hydroxyethyl methacrylate (poly-HEMA).
Methods.
tSKPs generated from our adherent monolayer culture system were investigated for protein expression and differentiation capacity.
The aggregated cells and the proliferative cells within tSKP spheres were detected by mix-culturing FBs expressing two different fluorescent proteins and BrdU- or EdU-positive cells, respectively.
To distinguish tSKPs from FBs, we compared their phenotypes and transcriptomes.
The tumorigenicity of tSKPs and FBs was also assessed in our study.
Results.
tSKPs expressed Versican, Fibronectin, Vimentin, Sox2, and Nestin.
Under appropriate stimuli, tSKPs could differentiate to mesenchymal or neural lineages.
While these spheres were heterogeneous populations consisting of both proliferative and aggregated cells, the rate of proliferative cells correlated with a seeding density.
tSKPs, isolated from FBs, were distinctive from FBs in cell cycle, marker expression, neural differentiation potential, and transcript profiles despite the two sharing partial similarity in certain properties.
As for tumorigenesis, both tSKPs and FBs could be considered as nontumorigenic ex vivo and in vivo.
Conclusion.
tSKPs were heterogeneous populations presenting similar characteristics as traditional SKPs, while being different from FBs.
The potential mixture of FBs in spheres did not affect the biosafety of tSKPs, as both of which had normal karyotype and nontumorigenicity.
Taken together, we suggested tSKPs had potential applications in regenerative medicine.
American Psychological Association (APA)
Dai, Ru& Hua, Wei& Chen, Wei& Xiong, Lidan& Li, Li& Li, Yiming. 2019. Isolation, Characterization, and Safety Evaluation of Human Skin-Derived Precursors from an Adherent Monolayer Culture System. Stem Cells International،Vol. 2019, no. 2019, pp.1-22.
https://search.emarefa.net/detail/BIM-1209928
Modern Language Association (MLA)
Dai, Ru…[et al.]. Isolation, Characterization, and Safety Evaluation of Human Skin-Derived Precursors from an Adherent Monolayer Culture System. Stem Cells International No. 2019 (2019), pp.1-22.
https://search.emarefa.net/detail/BIM-1209928
American Medical Association (AMA)
Dai, Ru& Hua, Wei& Chen, Wei& Xiong, Lidan& Li, Li& Li, Yiming. Isolation, Characterization, and Safety Evaluation of Human Skin-Derived Precursors from an Adherent Monolayer Culture System. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-22.
https://search.emarefa.net/detail/BIM-1209928
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209928