Human Menstrual Blood-Derived Stem Cells Inhibit the Proliferation of HeLa Cells via TGF-β1-Mediated JNKP21 Signaling Pathways
Joint Authors
Liu, Quanwen
Liu, Qian-Yu
Ruan, Feng
Li, Jing-Yuan
Hu, Ping
Chen, Hou-Wen
Liu, Quan-Wen
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-19
Country of Publication
Egypt
No. of Pages
18
Abstract EN
Human menstrual blood-derived stem cells (hMBSCs) are a novel type of mesenchymal stem cells (MSCs) that have a high proliferative rate, multilineage differentiation potential, low immunogenicity, and low oncogenicity, making them suitable candidates for regenerative medicine.
The therapeutic efficacy of hMBSCs has been demonstrated in some diseases; however, their effects on cervical cancer remain unclear.
In the present study, we investigated whether hMBSCs have anticancer properties on cervical cancer cells in vivo and in vitro, which has not yet been reported.
In vitro, transwell coculturing experiments revealed that hMBSCs suppress the proliferation and invasion of HeLa cervical cancer cells by inducing G0/G1 cell cycle arrest.
In vivo, we established a xenografted BALB/c nude mouse model by subcutaneously coinjecting HeLa cells with hMBSCs for 21 days.
We found that hMBSCs significantly decrease the average volume and average weight of xenografted tumors.
ELISA, TGF-β1 antibody, and recombinant human TGF-β1 (rhTGF-β1) were used to analyze whether TGF-β1 contributed to cell cycle arrest.
We found that hMBSC-secreted TGF-β1 and rhTGF-β1 induced cell cycle arrest and increased the expression of phospho-JNK and phospho-P21 in HeLa cells, which was mostly reversed by TGF-β1 antibody.
These results indicate that hMBSCs have antitumor properties on cervical cancer in vitro and in vivo, mediated by the TGF-β1/JNK/p21 signaling pathway.
In conclusion, this study suggests that hMBSC-based therapy is promising for the treatment of cervical cancer.
American Psychological Association (APA)
Liu, Qian-Yu& Ruan, Feng& Li, Jing-Yuan& Liu, Quanwen& Hu, Ping& Chen, Hou-Wen…[et al.]. 2019. Human Menstrual Blood-Derived Stem Cells Inhibit the Proliferation of HeLa Cells via TGF-β1-Mediated JNKP21 Signaling Pathways. Stem Cells International،Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1209956
Modern Language Association (MLA)
Liu, Qian-Yu…[et al.]. Human Menstrual Blood-Derived Stem Cells Inhibit the Proliferation of HeLa Cells via TGF-β1-Mediated JNKP21 Signaling Pathways. Stem Cells International No. 2019 (2019), pp.1-18.
https://search.emarefa.net/detail/BIM-1209956
American Medical Association (AMA)
Liu, Qian-Yu& Ruan, Feng& Li, Jing-Yuan& Liu, Quanwen& Hu, Ping& Chen, Hou-Wen…[et al.]. Human Menstrual Blood-Derived Stem Cells Inhibit the Proliferation of HeLa Cells via TGF-β1-Mediated JNKP21 Signaling Pathways. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1209956
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1209956