Uniaxial Cyclic Tensile Stretching at 8% Strain Exclusively Promotes Tenogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells
Joint Authors
Abbas, Azlina Amir
Pingguan-Murphy, Belinda
Kamarul, Tunku
Nam, Hui Yin
Merican, Azhar Mahmood
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-21
Country of Publication
Egypt
No. of Pages
16
Abstract EN
The present study was conducted to establish the amount of mechanical strain (uniaxial cyclic stretching) required to provide optimal tenogenic differentiation expression in human mesenchymal stromal cells (hMSCs) in vitro, in view of its potential application for tendon maintenance and regeneration.
Methods.
In the present study, hMSCs were subjected to 1 Hz uniaxial cyclic stretching for 6, 24, 48, and 72 hours; and were compared to unstretched cells.
Changes in cell morphology were observed under light and atomic force microscopy.
The tenogenic, osteogenic, adipogenic, and chondrogenic differentiation potential of hMSCs were evaluated using biochemical assays, extracellular matrix expressions, and selected mesenchyme gene expression markers; and were compared to primary tenocytes.
Results.
Cells subjected to loading displayed cytoskeletal coarsening, longer actin stress fiber, and higher cell stiffness as early as 6 hours.
At 8% and 12% strains, an increase in collagen I, collagen III, fibronectin, and N-cadherin production was observed.
Tenogenic gene expressions were highly expressed (p<0.05) at 8% (highest) and 12%, both comparable to tenocytes.
In contrast, the osteoblastic, chondrogenic, and adipogenic marker genes appeared to be downregulated.
Conclusion.
Our study suggests that mechanical loading at 8% strain and 1 Hz provides exclusive tenogenic differentiation; and produced comparable protein and gene expression to primary tenocytes.
American Psychological Association (APA)
Nam, Hui Yin& Pingguan-Murphy, Belinda& Abbas, Azlina Amir& Merican, Azhar Mahmood& Kamarul, Tunku. 2019. Uniaxial Cyclic Tensile Stretching at 8% Strain Exclusively Promotes Tenogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1210071
Modern Language Association (MLA)
Nam, Hui Yin…[et al.]. Uniaxial Cyclic Tensile Stretching at 8% Strain Exclusively Promotes Tenogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells. Stem Cells International No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1210071
American Medical Association (AMA)
Nam, Hui Yin& Pingguan-Murphy, Belinda& Abbas, Azlina Amir& Merican, Azhar Mahmood& Kamarul, Tunku. Uniaxial Cyclic Tensile Stretching at 8% Strain Exclusively Promotes Tenogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1210071
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1210071