Repulsive Environment Attenuation during Adult Mouse Optic Nerve Regeneration
Joint Authors
Goulart, Camila Oliveira
Mendonça, Henrique Rocha
Oliveira, Julia Teixeira
Savoldi, Laura Maria
dos Santos Heringer, Luiza
dos Santos Rodrigues, Alexandre
Paes-de-Carvalho, Roberto
Martinez, Ana Maria Blanco
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The regenerative capacity of CNS tracts has ever been a great hurdle to regenerative medicine.
Although recent studies have described strategies to stimulate retinal ganglion cells (RGCs) to regenerate axons through the optic nerve, it still remains to be elucidated how these therapies modulate the inhibitory environment of CNS.
Thus, the present work investigated the environmental content of the repulsive axon guidance cues, such as Sema3D and its receptors, myelin debris, and astrogliosis, within the regenerating optic nerve of mice submitted to intraocular inflammation + cAMP combined to conditional deletion of PTEN in RGC after optic nerve crush.
We show here that treatment was able to promote axonal regeneration through the optic nerve and reach visual targets at twelve weeks after injury.
The Regenerating group presented reduced MBP levels, increased microglia/macrophage number, and reduced astrocyte reactivity and CSPG content following optic nerve injury.
In addition, Sema3D content and its receptors are reduced in the Regenerating group.
Together, our results provide, for the first time, evidence that several regenerative repulsive signals are reduced in regenerating optic nerve fibers following a combined therapy.
Therefore, the treatment used made the CNS microenvironment more permissive to regeneration.
American Psychological Association (APA)
Goulart, Camila Oliveira& Mendonça, Henrique Rocha& Oliveira, Julia Teixeira& Savoldi, Laura Maria& dos Santos Heringer, Luiza& dos Santos Rodrigues, Alexandre…[et al.]. 2018. Repulsive Environment Attenuation during Adult Mouse Optic Nerve Regeneration. Neural Plasticity،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1210195
Modern Language Association (MLA)
Goulart, Camila Oliveira…[et al.]. Repulsive Environment Attenuation during Adult Mouse Optic Nerve Regeneration. Neural Plasticity No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1210195
American Medical Association (AMA)
Goulart, Camila Oliveira& Mendonça, Henrique Rocha& Oliveira, Julia Teixeira& Savoldi, Laura Maria& dos Santos Heringer, Luiza& dos Santos Rodrigues, Alexandre…[et al.]. Repulsive Environment Attenuation during Adult Mouse Optic Nerve Regeneration. Neural Plasticity. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1210195
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1210195