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Gallic Acid-L-Leucine Conjugate Protects Mice against LPS-Induced Inflammation and Sepsis via Correcting Proinflammatory Lipid Mediator Profiles and Oxidative Stress
Joint Authors
Cheng, Yuanyuan
Tse, Hung-Fat
Rong, Jianhui
Li, Xuechen
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-25
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The pathology of endotoxin LPS-induced sepsis is hallmarked by aberrant production of proinflammatory lipid mediators and nitric oxide (NO).
The aim of the present study was to determine whether the new product gallic acid-L-leucine (GAL) conjugate could ameliorate the LPS-induced dysregulation of arachidonic acid metabolism and NO production.
We first investigated the effects of GAL conjugate on the expression of proinflammatory enzymes and the production of proinflammatory NO and lipid mediators in mouse macrophage cell line RAW264.7, primary peritoneal macrophages, and mouse model.
Western blot analyses revealed that GAL attenuated LPS-induced expression of iNOS, COX-2, and 5-LOX in a concentration-dependent manner.
Consistently, probing NO-mediated fluorescence revealed that GAL antagonized the stimulatory effect of LPS on iNOS activity.
By profiling of lipid mediators with ESI-MS-based lipidomics, we found that GAL suppressed LPS-induced overproduction of prostaglandin E2, prostaglandin F2, leukotriene B4, and thromboxane B2.
We further discovered that GAL might exhibit anti-inflammatory activities by the following mechanisms: (1) suppressing LPS-induced activation of MAP kinases (i.e., ERK1/2, JNK, and p38); (2) reducing the production of reactive oxygen species (ROS); and (3) preventing LPS-induced nuclear translocation of transcription factors NF-κB and AP-1.
Consequently, GAL significantly decreased the levels of COX-2 and iNOS expression and the plasma levels of proinflammatory lipid mediators in LPS-treated mice.
GAL pretreatment enhanced the survival of mice against LPS-induced endotoxic shock.
Taken together, our results suggest that GAL may be a potential anti-inflammatory drug for the treatment of endotoxemia and sepsis.
American Psychological Association (APA)
Cheng, Yuanyuan& Li, Xuechen& Tse, Hung-Fat& Rong, Jianhui. 2018. Gallic Acid-L-Leucine Conjugate Protects Mice against LPS-Induced Inflammation and Sepsis via Correcting Proinflammatory Lipid Mediator Profiles and Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1210820
Modern Language Association (MLA)
Cheng, Yuanyuan…[et al.]. Gallic Acid-L-Leucine Conjugate Protects Mice against LPS-Induced Inflammation and Sepsis via Correcting Proinflammatory Lipid Mediator Profiles and Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1210820
American Medical Association (AMA)
Cheng, Yuanyuan& Li, Xuechen& Tse, Hung-Fat& Rong, Jianhui. Gallic Acid-L-Leucine Conjugate Protects Mice against LPS-Induced Inflammation and Sepsis via Correcting Proinflammatory Lipid Mediator Profiles and Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1210820
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1210820