Intermittent Hypoxia Increases the Severity of Bleomycin-Induced Lung Injury in Mice
Joint Authors
Gille, Thomas
Didier, Morgane
Rotenberg, Cécile
Delbrel, Eva
Marchant, Dominique
Sutton, Angela
Dard, Nicolas
Haine, Liasmine
Voituron, Nicolas
Bernaudin, Jean-François
Valeyre, Dominique
Nunes, Hilario
Besnard, Valérie
Boncoeur, Emilie
Planès, Carole
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-14
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background.
Severe obstructive sleep apnea (OSA) with chronic intermittent hypoxia (IH) is common in idiopathic pulmonary fibrosis (IPF).
Here, we evaluated the impact of IH on bleomycin- (BLM-) induced pulmonary fibrosis in mice.
Methods.
C57BL/6J mice received intratracheal BLM or saline and were exposed to IH (40 cycles/hour; FiO2 nadir: 6%; 8 hours/day) or intermittent air (IA).
In the four experimental groups, we evaluated (i) survival; (ii) alveolar inflammation, pulmonary edema, lung oxidative stress, and antioxidant enzymes; (iii) lung cell apoptosis; and (iv) pulmonary fibrosis.
Results.
Survival at day 21 was lower in the BLM-IH group (p<0.05).
Pulmonary fibrosis was more severe at day 21 in BLM-IH mice, as assessed by lung collagen content (p=0.02) and histology.
At day 4, BLM-IH mice developed a more severe neutrophilic alveolitis, (p<0.001).
Lung oxidative stress was observed, and superoxide dismutase and glutathione peroxidase expression was decreased in BLM-IH mice (p<0.05 versus BLM-IA group).
At day 8, pulmonary edema was observed and lung cell apoptosis was increased in the BLM-IH group.
Conclusion.
These results show that exposure to chronic IH increases mortality, lung inflammation, and lung fibrosis in BLM-treated mice.
This study raises the question of the worsening impact of severe OSA in IPF patients.
American Psychological Association (APA)
Gille, Thomas& Didier, Morgane& Rotenberg, Cécile& Delbrel, Eva& Marchant, Dominique& Sutton, Angela…[et al.]. 2018. Intermittent Hypoxia Increases the Severity of Bleomycin-Induced Lung Injury in Mice. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1210834
Modern Language Association (MLA)
Gille, Thomas…[et al.]. Intermittent Hypoxia Increases the Severity of Bleomycin-Induced Lung Injury in Mice. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1210834
American Medical Association (AMA)
Gille, Thomas& Didier, Morgane& Rotenberg, Cécile& Delbrel, Eva& Marchant, Dominique& Sutton, Angela…[et al.]. Intermittent Hypoxia Increases the Severity of Bleomycin-Induced Lung Injury in Mice. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1210834
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1210834