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Losartan Ameliorates Calcium Oxalate-Induced Elevation of Stone-Related Proteins in Renal Tubular Cells by Inhibiting NADPH Oxidase and Oxidative Stress
Joint Authors
Wang, Shaogang
Zhang, Jiaqiao
Wang, Qing
Lu, Yuchao
Hu, Henglong
Qin, Baolong
Wang, Yufeng
Li, Cong
Zhu, Jianning
Zhu, Yunpeng
Xun, Yang
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Calcium oxalate (CaOx) is the most common type of urinary stone.
Increase of ROS and NADPH oxidase gives rise to inflammation and injury of renal tubular cells, which promotes CaOx stone formation.
Recent studies have revealed that the renin-angiotensin system might play a role in kidney crystallization and ROS production.
Here, we investigated the involvement of Ang II/AT1R and losartan in CaOx stone formation.
NRK-52E cells were incubated with CaOx crystals, and glyoxylic acid-induced hyperoxaluric rats were treated with losartan.
Oxidative stress statuses were evaluated by detection of ROS, oxidative products (8-OHdG and MDA), and antioxidant enzymes (SOD and CAT).
Expression of NADPH oxidase subunits (Nox2 and Nox4), NF-κB pathway subunits (p50 and p65), and stone-related proteins such as OPN, CD44, and MCP-1 was determined by Western blotting.
The results revealed upregulation of Ang II/AT1R by CaOx treatment.
CaOx-induced ROS and stone-related protein upregulation were mediated by the Ang II/AT1R signaling pathway.
Losartan ameliorated renal tubular cell expression of stone-related proteins and renal crystallization by inhibiting NADPH oxidase and oxidative stress.
We conclude that losartan might be a promising preventive and therapeutic candidate for hyperoxaluria nephrolithiasis.
American Psychological Association (APA)
Qin, Baolong& Wang, Qing& Lu, Yuchao& Li, Cong& Hu, Henglong& Zhang, Jiaqiao…[et al.]. 2018. Losartan Ameliorates Calcium Oxalate-Induced Elevation of Stone-Related Proteins in Renal Tubular Cells by Inhibiting NADPH Oxidase and Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1210842
Modern Language Association (MLA)
Qin, Baolong…[et al.]. Losartan Ameliorates Calcium Oxalate-Induced Elevation of Stone-Related Proteins in Renal Tubular Cells by Inhibiting NADPH Oxidase and Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1210842
American Medical Association (AMA)
Qin, Baolong& Wang, Qing& Lu, Yuchao& Li, Cong& Hu, Henglong& Zhang, Jiaqiao…[et al.]. Losartan Ameliorates Calcium Oxalate-Induced Elevation of Stone-Related Proteins in Renal Tubular Cells by Inhibiting NADPH Oxidase and Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1210842
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1210842