PKM2 Is Required to Activate Myeloid Dendritic Cells from Patients with Severe Aplastic Anemia
Joint Authors
Wang, Huaquan
Liu, Chunyan
Wang, Ting
Fu, Rong
Shao, Zonghong
Zheng, Mengying
Jiang, Huijuan
Ding, Kai
Zhou, Qiufan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Severe aplastic anemia (SAA) is an autoimmune disease in which bone marrow failure is mediated by activated myeloid dendritic cells (mDCs) and T lymphocytes.
Recent research has identified a strong immunomodulatory effect of pyruvate kinase M2 (PKM2) on dendritic cells in immune-mediated diseases.
In this study, we aimed to explore the role of PKM2 in the activation of mDCs in SAA.
We observed conspicuously higher levels of PKM2 in mDCs from SAA patients compared to normal controls at both the gene and protein levels.
Concurrently, we unexpectedly discovered that after the mDC-specific downregulation of PKM2, mDCs from patients with SAA exhibited weakened phagocytic activity and significantly decreased and shortened dendrites relative to their counterparts from normal controls.
The expression levels of the costimulatory molecules CD86 and CD80 were also reduced on mDCs.
Our results also suggested that PKM2 knockdown in mDCs reduced the abilities of these cells to promote the activation of CD8+ T cells (CTLs), leading to the decreased secretion of cytotoxic factors by the latter cell type.
These findings demonstrate that mDC activation requires an elevated intrinsic PKM2 level and that PKM2 improves the immune status of patients with SAA by enhancing the functions of mDCs and, consequently, CTLs.
American Psychological Association (APA)
Liu, Chunyan& Zheng, Mengying& Wang, Ting& Jiang, Huijuan& Fu, Rong& Wang, Huaquan…[et al.]. 2018. PKM2 Is Required to Activate Myeloid Dendritic Cells from Patients with Severe Aplastic Anemia. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1210869
Modern Language Association (MLA)
Liu, Chunyan…[et al.]. PKM2 Is Required to Activate Myeloid Dendritic Cells from Patients with Severe Aplastic Anemia. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1210869
American Medical Association (AMA)
Liu, Chunyan& Zheng, Mengying& Wang, Ting& Jiang, Huijuan& Fu, Rong& Wang, Huaquan…[et al.]. PKM2 Is Required to Activate Myeloid Dendritic Cells from Patients with Severe Aplastic Anemia. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1210869
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1210869