Epigallocatechin Gallate Attenuates Bladder Dysfunction via Suppression of Oxidative Stress in a Rat Model of Partial Bladder Outlet Obstruction
Joint Authors
Liu, Chong
Chen, Qi
Zhou, Juan
Wang, Zhong
Wan, Xiang
Gu, Meng
Yang, Tianye
Chen, Yanbo
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Purpose.
To investigate the protective effect of epigallocatechin gallate (EGCG), a green tea extract, and its underlying mechanism on bladder dysfunction in a rat model of bladder outlet obstruction (BOO).
Materials and Methods.
Sprague-Dawley rats of BOO were surgically induced and followed by treatment with EGCG (5 mg/kg/day) or saline (control) via intraperitoneal injection.
Cystometry was performed on four weeks postoperatively in conscious rats.
H&E, Masson trichrome, and TUNEL staining were performed to observe tissue alterations.
Oxidative stress markers were measured, and protein expression of Nrf2-ARE pathway was examined by immunohistochemistry and Western blotting.
Results.
Our data showed that EGCG could increase the peak voiding pressure and bladder compliance and prolong micturition interval of BOO rats compared with control and finally reduce the frequency of urinary.
EGCG could ameliorate the increase of collagen fibers and ROS induced by obstruction and increase the activity of SOD, GSH-Px, and CAT.
The level of cell apoptosis was decreased in BOO rats treated with EGCG compared with control, and caspase-3 expression was reduced as well.
Moreover, EGCG could activate the Nrf2 expression with elevation of its target antioxidant proteins.
Conclusions.
EGCG alleviates BOO-induced bladder dysfunction via suppression of oxidative stress and activation of the protein expression of Nrf2-ARE pathway.
American Psychological Association (APA)
Gu, Meng& Liu, Chong& Wan, Xiang& Yang, Tianye& Chen, Yanbo& Zhou, Juan…[et al.]. 2018. Epigallocatechin Gallate Attenuates Bladder Dysfunction via Suppression of Oxidative Stress in a Rat Model of Partial Bladder Outlet Obstruction. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1210873
Modern Language Association (MLA)
Gu, Meng…[et al.]. Epigallocatechin Gallate Attenuates Bladder Dysfunction via Suppression of Oxidative Stress in a Rat Model of Partial Bladder Outlet Obstruction. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1210873
American Medical Association (AMA)
Gu, Meng& Liu, Chong& Wan, Xiang& Yang, Tianye& Chen, Yanbo& Zhou, Juan…[et al.]. Epigallocatechin Gallate Attenuates Bladder Dysfunction via Suppression of Oxidative Stress in a Rat Model of Partial Bladder Outlet Obstruction. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1210873
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1210873