Icariin Improves the Viability and Function of Cryopreserved Human Nucleus Pulposus-Derived Mesenchymal Stem Cells
Joint Authors
Shao, Zengwu
Deng, Xiangyu
Huang, Donghua
Chen, Sheng
Ma, Kaige
Zhao, Lei
Li, Zhiliang
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-27
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Nucleus pulposus-derived mesenchymal stem cells (NPMSCs) have shown a good prospect in the regeneration of intervertebral disc (IVD) tissues.
However, fresh NPMSCs are not always readily available for basic research and clinical applications.
Therefore, there is a need for an effective long-term cryopreservation method for NPMSCs.
The aim of this study was to determine whether adding icariin (ICA) to the conventional cryoprotectant containing dimethyl sulfoxide (DMSO) had a better cryoprotective effect for NPMSCs.
The results showed that the freezing solution containing ICA along with DMSO significantly increased the postthawed cell viability, decreased the apoptosis rate, improved cell adherence, and maintained the mitochondrial functions, as compared to the freezing solution containing DMSO alone.
And the inhibition of oxidative stress and upregulation of heat shock proteins (HSPs) in the presence of ICA also confirmed the beneficial effect of ICA.
Furthermore, ICA had no cytotoxicity and did not alter the characteristics of postthawed NPMSCs.
In conclusion, these results suggested that the addition of ICA to the conventional freezing medium could improve the viability and function of the cryopreserved human NPMSCs and provided an optimal formulated freezing solution for human NPMSC cryopreservation.
American Psychological Association (APA)
Chen, Sheng& Deng, Xiangyu& Ma, Kaige& Zhao, Lei& Huang, Donghua& Li, Zhiliang…[et al.]. 2018. Icariin Improves the Viability and Function of Cryopreserved Human Nucleus Pulposus-Derived Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211237
Modern Language Association (MLA)
Chen, Sheng…[et al.]. Icariin Improves the Viability and Function of Cryopreserved Human Nucleus Pulposus-Derived Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1211237
American Medical Association (AMA)
Chen, Sheng& Deng, Xiangyu& Ma, Kaige& Zhao, Lei& Huang, Donghua& Li, Zhiliang…[et al.]. Icariin Improves the Viability and Function of Cryopreserved Human Nucleus Pulposus-Derived Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211237
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211237