GRP78 Promotes Neural Stem Cell Antiapoptosis and Survival in Response to Oxygen-Glucose Deprivation (OGD)Reoxygenation through PI3KAkt, ERK12, and NF-κBp65 Pathways
Joint Authors
Liu, Xinfeng
Liu, Qian
Li, Yun
Li, Yunzi
Zhou, Lin
Xu, Pengfei
Li, Juanji
Liu, Xiaoyun
Lv, Qiushi
Guo, Hongquan
Cai, Haodi
Sun, Rui
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-10
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
When brain injury happens, endogenous neural stem cells (NSCs) located in the adult subventricular zone (SVZ) and subgranular zone (SGZ) are attacked by ischemia/reperfusion to undergo cellular apoptosis and death before being induced to migrate to the lesion point and differentiate into mature neural cells for damaged cell replacement.
Although promoting antiapoptosis and NSC survival are critical to neuroregeneration, the mechanism has yet been elucidated clearly.
Here in this study, we established an in vitro oxygen-glucose deprivation (OGD)/reoxygenation model on NSCs and detected glucose-regulated protein 78 (GRP78) involved in apoptosis, while in the absence of GRP78 by siRNA transfection, OGD/reoxygenation triggered PI3K/Akt, ERK1/2, and NF-κB/p65 activation, and induced NSC apoptosis was attenuated.
Further investigation, respectively, with the inhibitor of PI3K/Akt or ERK1/2 demonstrated a blockage on GRP78 upregulation, while the inhibition of NF-κB rarely affected GRP78 induction by OGD/reoxygenation.
The results indicated the bidirectional regulations of GRP78-PI3K/Akt and GRP78-ERK1/2 and the one-way signalling transduction through GRP78 to NF-κB/p65 on NSC survival from OGD/reoxygenation.
In conclusion, we found that GRP78 mediated the signalling cross talk through PI3K/Akt, ERK1/2, and NF-κB/p65, which leads to antiapoptosis and NSC survival from ischemic stroke.
Our finding gives a new evidence of GRP78 in NSCs as well as a new piece of signalling mechanism elucidation to NSC survival from ischemic stroke.
American Psychological Association (APA)
Liu, Qian& Li, Yun& Zhou, Lin& Li, Yunzi& Xu, Pengfei& Liu, Xiaoyun…[et al.]. 2018. GRP78 Promotes Neural Stem Cell Antiapoptosis and Survival in Response to Oxygen-Glucose Deprivation (OGD)Reoxygenation through PI3KAkt, ERK12, and NF-κBp65 Pathways. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211263
Modern Language Association (MLA)
Liu, Qian…[et al.]. GRP78 Promotes Neural Stem Cell Antiapoptosis and Survival in Response to Oxygen-Glucose Deprivation (OGD)Reoxygenation through PI3KAkt, ERK12, and NF-κBp65 Pathways. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1211263
American Medical Association (AMA)
Liu, Qian& Li, Yun& Zhou, Lin& Li, Yunzi& Xu, Pengfei& Liu, Xiaoyun…[et al.]. GRP78 Promotes Neural Stem Cell Antiapoptosis and Survival in Response to Oxygen-Glucose Deprivation (OGD)Reoxygenation through PI3KAkt, ERK12, and NF-κBp65 Pathways. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211263
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211263