DUOX1 Silencing in Mammary Cell Alters the Response to Genotoxic Stress
Joint Authors
Hecht, Fabio
Fortunato, Rodrigo S.
Carvalho, Denise P.
Gomes, Luciana R.
Munford, Veridiana
Pessoa, Carolina Fittipaldi
Quinet, Annabel
Kajitani, Gustavo S.
Milito, Cristiane Bedran
Menck, Carlos Frederico Martins
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
DUOX1 is an H2O2-generating enzyme related to a wide range of biological features, such as hormone synthesis, host defense, cellular proliferation, and fertilization.
DUOX1 is frequently downregulated in lung and liver cancers, suggesting a tumor suppressor role for this enzyme.
Here, we show that DUOX1 expression is decreased in breast cancer cell lines and also in breast cancers when compared to the nontumor counterpart.
In order to address the role of DUOX1 in breast cells, we stably knocked down the expression of DUOX1 in nontumor mammary cells (MCF12A) with shRNA.
This led to higher cell proliferation rates and decreased migration and adhesion properties, which are typical features for transformed cells.
After genotoxic stress induced by doxorubicin, DUOX1-silenced cells showed reduced IL-6 and IL-8 secretion and increased apoptosis levels.
Furthermore, the cell proliferation rate was higher in DUOX1-silenced cells after doxorubicin medication in comparison to control cells.
In conclusion, we demonstrate here that DUOX1 is silenced in breast cancer, which seems to be involved in breast carcinogenesis.
American Psychological Association (APA)
Fortunato, Rodrigo S.& Gomes, Luciana R.& Munford, Veridiana& Pessoa, Carolina Fittipaldi& Quinet, Annabel& Hecht, Fabio…[et al.]. 2018. DUOX1 Silencing in Mammary Cell Alters the Response to Genotoxic Stress. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1211270
Modern Language Association (MLA)
Fortunato, Rodrigo S.…[et al.]. DUOX1 Silencing in Mammary Cell Alters the Response to Genotoxic Stress. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1211270
American Medical Association (AMA)
Fortunato, Rodrigo S.& Gomes, Luciana R.& Munford, Veridiana& Pessoa, Carolina Fittipaldi& Quinet, Annabel& Hecht, Fabio…[et al.]. DUOX1 Silencing in Mammary Cell Alters the Response to Genotoxic Stress. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1211270
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211270