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Tanshinone IIA Pretreatment Protects H9c2 Cells against AnoxiaReoxygenation Injury: Involvement of the Translocation of Bcl-2 to Mitochondria Mediated by 14-3-3η
Joint Authors
He, Ming
He, Huan
Huang, Jiyi
Wu, Zelong
Yin, Dong
Qiao, Yang
Zhang, Zeyu
Xu, Ping
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-28
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Tanshinone IIA is an important component that is isolated from danshen (Salvia miltiorrhiza), which is known to be beneficial for cardiovascular health.
In this study, we determined the effects of Tanshinone IIA and its underlying mechanisms of action in an anoxia/reoxygenation (A/R) cell line model.
Prior to inducing A/R injury, rat cardiomyocyte-derived cell line H9c2 was stimulated with 8 μM of Tanshinone IIA for 48 hours.
When compared with the A/R group, the Tanshinone IIA treatment significantly increased cell viability and decreased lactate dehydrogenase activity.
Tanshinone IIA upregulated 14-3-3η expression and facilitated Bcl-2 translocation to the mitochondrial outer membrane, which bound with voltage-dependent anion channel 1.
In addition, pretreatment with Tanshinone IIA reduced the generation of reactive oxygen species and cytochrome c release, inactivated caspase-3, prevented mitochondrial permeability transition pore opening, and reduced the percentage of apoptotic cells.
Moreover, treatment with Tanshinone IIA reduced the level of malondialdehyde, thereby increasing the activity of superoxide dismutase and glutathione peroxidase.
Silencing the expression of 14-3-3η by adenovirus blocked the above-mentioned results.
These novel findings showed that pretreatment with Tanshinone IIA alleviated H9c2 cell damage against A/R injury and was associated with upregulation of 14-3-3η, thereby facilitating Bcl-2 translocation to the mitochondrial outer membrane and preventing mitochondrial permeability transition pore opening, decreasing cytochrome c release, preventing caspase-3 activation, and restraining apoptosis.
American Psychological Association (APA)
Zhang, Zeyu& He, Huan& Qiao, Yang& Huang, Jiyi& Wu, Zelong& Xu, Ping…[et al.]. 2018. Tanshinone IIA Pretreatment Protects H9c2 Cells against AnoxiaReoxygenation Injury: Involvement of the Translocation of Bcl-2 to Mitochondria Mediated by 14-3-3η. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1211273
Modern Language Association (MLA)
Zhang, Zeyu…[et al.]. Tanshinone IIA Pretreatment Protects H9c2 Cells against AnoxiaReoxygenation Injury: Involvement of the Translocation of Bcl-2 to Mitochondria Mediated by 14-3-3η. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1211273
American Medical Association (AMA)
Zhang, Zeyu& He, Huan& Qiao, Yang& Huang, Jiyi& Wu, Zelong& Xu, Ping…[et al.]. Tanshinone IIA Pretreatment Protects H9c2 Cells against AnoxiaReoxygenation Injury: Involvement of the Translocation of Bcl-2 to Mitochondria Mediated by 14-3-3η. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1211273
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211273