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Counteraction of Oxidative Stress by Vitamin E Affects Epigenetic Regulation by Increasing Global Methylation and Gene Expression of MLH1 and DNMT1 Dose Dependently in Caco-2 Cells
Joint Authors
Wagner, Karl-Heinz
Haslberger, Alexander G.
Zappe, Katja
Pointner, Angelika
Switzeny, Olivier J.
Magnet, Ulrich
Tomeva, Elena
Heller, Jutta
Mare, George
Knasmuller, S.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-22
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Obesity- or diabetes-induced oxidative stress is discussed as a major risk factor for DNA damage.
Vitamin E and many polyphenols exhibit antioxidative activities with consequences on epigenetic regulation of inflammation and DNA repair.
The present study investigated the counteraction of oxidative stress by vitamin E in the colorectal cancer cell line Caco-2 under normal (1 g/l) and high (4.5 g/l) glucose cell culture condition.
Malondialdehyde (MDA) as a surrogate marker of lipid peroxidation and reactive oxygen species (ROS) was analyzed.
Gene expression and promoter methylation of the DNA repair gene MutL homolog 1 (MLH1) and the DNA methyltransferase 1 (DNMT1) as well as global methylation by LINE-1 were investigated.
Results revealed a dose-dependent counteracting effect of vitamin E on H2O2-induced oxidative stress.
Thereby, 10 μM vitamin E proved to be more efficient than did 50 μM in reducing MDA.
Further, an induction of MLH1 and DNMT1 gene expression was noticed, accompanied by an increase in global methylation.
Whether LINE-1 hypomethylation is a cause or effect of oxidative stress is still unclear.
In conclusion, supplementation of exogenous antioxidants like vitamin E in vitro exhibits beneficial effects concerning oxidative stress as well as epigenetic regulation involved in DNA repair.
American Psychological Association (APA)
Zappe, Katja& Pointner, Angelika& Switzeny, Olivier J.& Magnet, Ulrich& Tomeva, Elena& Heller, Jutta…[et al.]. 2018. Counteraction of Oxidative Stress by Vitamin E Affects Epigenetic Regulation by Increasing Global Methylation and Gene Expression of MLH1 and DNMT1 Dose Dependently in Caco-2 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1211296
Modern Language Association (MLA)
Zappe, Katja…[et al.]. Counteraction of Oxidative Stress by Vitamin E Affects Epigenetic Regulation by Increasing Global Methylation and Gene Expression of MLH1 and DNMT1 Dose Dependently in Caco-2 Cells. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1211296
American Medical Association (AMA)
Zappe, Katja& Pointner, Angelika& Switzeny, Olivier J.& Magnet, Ulrich& Tomeva, Elena& Heller, Jutta…[et al.]. Counteraction of Oxidative Stress by Vitamin E Affects Epigenetic Regulation by Increasing Global Methylation and Gene Expression of MLH1 and DNMT1 Dose Dependently in Caco-2 Cells. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1211296
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211296