![](/images/graphics-bg.png)
Heme Oxygenase-1 May Affect Cell Signalling via Modulation of Ganglioside Composition
Joint Authors
Józkowicz, Alicja
Dulak, Jozef
Vítek, Libor
Kachamakova-Trojanowska, Neli
Jašprová, Jana
Muchová, Lucie
Šmíd, Václav
Šuk, Jakub
Valášková, Petra
Šmíd, František
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Heme oxygenase 1 (Hmox1), a ubiquitous enzyme degrading heme to carbon monoxide, iron, and biliverdin, is one of the cytoprotective enzymes induced in response to a variety of stimuli, including cellular oxidative stress.
Gangliosides, sialic acid-containing glycosphingolipids expressed in all cells, are involved in cell recognition, signalling, and membrane stabilization.
Their expression is often altered under many pathological and physiological conditions including cell death, proliferation, and differentiation.
The aim of this study was to assess the possible role of Hmox1 in ganglioside metabolism in relation to oxidative stress.
The content of liver and brain gangliosides, their cellular distribution, and mRNA as well as protein expression of key glycosyltransferases were determined in Hmox1 knockout mice as well as their wild-type littermates.
To elucidate the possible underlying mechanisms between Hmox1 and ganglioside metabolism, hepatoblastoma HepG2 and neuroblastoma SH-SY5Y cell lines were used for in vitro experiments.
Mice lacking Hmox1 exhibited a significant increase in concentrations of liver and brain gangliosides and in mRNA expression of the key enzymes of ganglioside metabolism.
A marked shift of GM1 ganglioside from the subsinusoidal part of the intracellular compartment into sinusoidal membranes of hepatocytes was shown in Hmox1 knockout mice.
Induction of oxidative stress by chenodeoxycholic acid in vitro resulted in a significant increase in GM3, GM2, and GD1a gangliosides in SH-SY5Y cells and GM3 and GM2 in the HepG2 cell line.
These changes were abolished with administration of bilirubin, a potent antioxidant agent.
These observations were closely related to oxidative stress-mediated changes in sialyltransferase expression regulated at least partially through the protein kinase C pathway.
We conclude that oxidative stress is an important factor modulating synthesis and distribution of gangliosides in vivo and in vitro which might affect ganglioside signalling in higher organisms.
American Psychological Association (APA)
Šmíd, Václav& Šuk, Jakub& Kachamakova-Trojanowska, Neli& Jašprová, Jana& Valášková, Petra& Józkowicz, Alicja…[et al.]. 2018. Heme Oxygenase-1 May Affect Cell Signalling via Modulation of Ganglioside Composition. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211314
Modern Language Association (MLA)
Šmíd, Václav…[et al.]. Heme Oxygenase-1 May Affect Cell Signalling via Modulation of Ganglioside Composition. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1211314
American Medical Association (AMA)
Šmíd, Václav& Šuk, Jakub& Kachamakova-Trojanowska, Neli& Jašprová, Jana& Valášková, Petra& Józkowicz, Alicja…[et al.]. Heme Oxygenase-1 May Affect Cell Signalling via Modulation of Ganglioside Composition. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211314
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211314