Calbindin-D28k in the Brain Influences the Expression of Cellular Prion Protein
Joint Authors
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The phenotypes of calbindin-D9k- (CaBP-9k-) knockout (KO), calbindin-D28k- (CaBP-28k-) KO, and CaBP-9k/28k-KO mice are similar to those of wild-type (WT) mice due to the compensatory action of other calcium transport proteins.
In this study, we investigated the expression of cellular prion protein (PrPC) in the brains of CaBP-9k-, CaBP-28k-, and CaBP-9k/28k-KO mice.
PrPC expression was significantly upregulated in the brain of all three strains.
Levels of phospho-Akt (Ser473) and phospho-Bad (Ser136) were significantly elevated, but those of phospho-ERK and phospho-Bad (Ser155 and 112) were significantly reduced in the brains of CaBP-9k-, CaBP-28k-, and CaBP-9k/28k-KO mice.
The expressions of the Bcl-2, p53, Bax, Cu/Zn-SOD, and Mn-SOD proteins were decreased in the brains of all KO mice.
Expression of the endoplasmic reticulum marker protein BiP/GRP78 was decreased, and that of the CHOP protein was increased in the brains of those KO mice.
To identify the roles of CaBP-28k, we transfected PC12 cells with siRNA for CaBP-28k and found increased expression of the PrPC protein compared to the levels in control cells.
These results suggest that CaBP-28k expression may regulate PrPC protein expression and these mice may be vulnerable to the influence of prion disease.
American Psychological Association (APA)
Yoo, Yeong-Min& Jeung, Eui-Bae. 2018. Calbindin-D28k in the Brain Influences the Expression of Cellular Prion Protein. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1211468
Modern Language Association (MLA)
Yoo, Yeong-Min& Jeung, Eui-Bae. Calbindin-D28k in the Brain Influences the Expression of Cellular Prion Protein. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1211468
American Medical Association (AMA)
Yoo, Yeong-Min& Jeung, Eui-Bae. Calbindin-D28k in the Brain Influences the Expression of Cellular Prion Protein. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1211468
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211468