Methane-Rich Saline Ameliorates Sepsis-Induced Acute Kidney Injury through Anti-Inflammation, Antioxidative, and Antiapoptosis Effects by Regulating Endoplasmic Reticulum Stress
Joint Authors
Zhang, Xinhua
Liu, Chang
Xiang, Xiaohong
Qu, Kai
Dong, Yanyan
Feng, Yang
Li, Zeyu
Jia, Yifan
Zhang, Jingyao
Cui, Ruixia
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Sepsis-induced acute kidney injury (AKI) is a severe complication of sepsis and an important cause of mortality in septic patients.
Previous investigations showed that methane had protective properties against different diseases in animal models.
This study is aimed at investigating whether methane-rich saline (MRS) has a protective effect against sepsis-induced AKI.
Sepsis was induced in wild-type C57BL/6 mice by cecal ligation and puncture (CLP), and the mice were divided into three groups: a sham control group (sham), a surgery group with saline intraperitoneal injection (i.p.) treatment (CLP + NS), and a surgery group with MRS i.p.
treatment (CLP + MRS).
24 h after the establishment of the sepsis, the blood and kidney tissues of mice in all groups were collected.
According to the serum levels of blood urea nitrogen (BUN) and creatinine (CRE) and a histologic analysis, which included hematoxylin-eosin (H&E) staining and periodic acid-Schiff (PAS) staining, MRS treatment protected renal function and tissues from acute injury.
Additionally, MRS treatment significantly ameliorated apoptosis, based on the levels of apoptosis-related protein makers, including cleaved caspase-3 and cleaved PARP, and the levels of Bcl-2/Bax expression and TUNEL staining.
In addition, the endoplasmic reticulum (ER) stress-related glucose-regulated protein 78 (GRP78)/activating transcription factor 4 (ATF4)/C/EBP homologous protein (CHOP)/caspase-12 apoptosis signaling pathway was significantly suppressed in the CLP + MRS group.
The levels of inflammation and oxidative stress were also reduced after MRS treatment.
These results showed that MRS has the potential to ameliorate sepsis-induced acute kidney injury through its anti-inflammatory, antioxidative, and antiapoptosis properties.
American Psychological Association (APA)
Jia, Yifan& Li, Zeyu& Feng, Yang& Cui, Ruixia& Dong, Yanyan& Zhang, Xinhua…[et al.]. 2018. Methane-Rich Saline Ameliorates Sepsis-Induced Acute Kidney Injury through Anti-Inflammation, Antioxidative, and Antiapoptosis Effects by Regulating Endoplasmic Reticulum Stress. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1211479
Modern Language Association (MLA)
Jia, Yifan…[et al.]. Methane-Rich Saline Ameliorates Sepsis-Induced Acute Kidney Injury through Anti-Inflammation, Antioxidative, and Antiapoptosis Effects by Regulating Endoplasmic Reticulum Stress. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1211479
American Medical Association (AMA)
Jia, Yifan& Li, Zeyu& Feng, Yang& Cui, Ruixia& Dong, Yanyan& Zhang, Xinhua…[et al.]. Methane-Rich Saline Ameliorates Sepsis-Induced Acute Kidney Injury through Anti-Inflammation, Antioxidative, and Antiapoptosis Effects by Regulating Endoplasmic Reticulum Stress. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1211479
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211479