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Phagocytosis of Candida albicans Inhibits Autophagic Flux in Macrophages
Joint Authors
Xu, Song
Chen, Xu
Duan, Zhimin
Chen, Qing
Du, Leilei
Tong, Jianbo
Zeng, Rong
Ma, Yuting
Li, Min
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-20
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Autophagy machinery has roles in the defense against microorganisms such as Candida albicans.
Lipidated LC3, the marker protein of autophagy, participates in the elimination of C.
albicans by forming a single-membrane phagosome; this process is called LC3-associated phagocytosis (LAP).
However, the influence of C.
albicans on autophagic flux is not clear.
In this study, we found that C.
albicans inhibited LC3 turnover in macrophages.
After the phagocytosis of C.
albicans in macrophages, we observed fewer acridine orange-positive vacuoles and RFP-GFP-LC3 puncta without colocalization with phagocytized C.
albicans.
However, phagocytosis of C.
albicans led to LC3 recruitment, but p62 and ATG9A did not colocalize with LC3 or C.
albicans.
These effects are due to an MTOR-independent pathway.
Nevertheless, we found that the C.
albicans pattern-associated molecular pattern β-glucan increased LC3 turnover.
In addition, phagocytosis of C.
albicans caused a decrease in BrdU incorporation.
Blocking autophagic flux aggravated this effect.
Our findings suggest that phagocytosis of C.
albicans decreases autophagic flux but induces LAP in an MTOR-independent manner in macrophages.
Occupation of LC3 by recruiting engulfed C.
albicans might contribute to the inhibition of autophagic flux.
Our study highlights the coordinated machinery between canonical autophagy and LAP that defends against C.
albicans challenge.
American Psychological Association (APA)
Duan, Zhimin& Chen, Qing& Du, Leilei& Tong, Jianbo& Xu, Song& Zeng, Rong…[et al.]. 2018. Phagocytosis of Candida albicans Inhibits Autophagic Flux in Macrophages. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1211526
Modern Language Association (MLA)
Duan, Zhimin…[et al.]. Phagocytosis of Candida albicans Inhibits Autophagic Flux in Macrophages. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1211526
American Medical Association (AMA)
Duan, Zhimin& Chen, Qing& Du, Leilei& Tong, Jianbo& Xu, Song& Zeng, Rong…[et al.]. Phagocytosis of Candida albicans Inhibits Autophagic Flux in Macrophages. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1211526
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211526