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Exosomes Derived from miR-214-Enriched Bone Marrow-Derived Mesenchymal Stem Cells Regulate Oxidative Damage in Cardiac Stem Cells by Targeting CaMKII
Joint Authors
Ge, Junbo
Deng, Wenwen
Long, Xianping
Zhao, Ranzun
Shi, Bei
Wang, Yan
Liu, Weiwei
Rong, Jidong
Liu, Debin
Xu, Guanxue
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-21, 21 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-07
Country of Publication
Egypt
No. of Pages
21
Main Subjects
Abstract EN
Cardiac stem cells (CSCs) have emerged as one of the most promising stem cells for cardiac protection.
Recently, exosomes from bone marrow-derived mesenchymal stem cells (BMSCs) have been found to facilitate cell proliferation and survival by transporting various bioactive molecules, including microRNAs (miRs).
In this study, we found that BMSC-derived exosomes (BMSC-exos) significantly decreased apoptosis rates and reactive oxygen species (ROS) production in CSCs after oxidative stress injury.
Moreover, a stronger effect was induced by exosomes collected from BMSCs cultured under hypoxic conditions (Hypoxic-exos) than those collected from BMSCs cultured under normal conditions (Nor-exos).
We also observed greater miR-214 enrichment in Hypoxic-exos than in Nor-exos.
In addition, a miR-214 inhibitor or mimics added to modulate miR-214 levels in BMSC-exos revealed that exosomes from miR-214-depleted BMSCs partially reversed the effects of hypoxia-induced exosomes on oxidative damage in CSCs.
These data further confirmed that miR-214 is the main effector molecule in BMSC-exos that protects CSCs from oxidative damage.
miR-214 mimic and inhibitor transfection assays verified that CaMKII is a target gene of miR-214 in CSCs, with exosome-pretreated CSCs exhibiting increased miR-214 levels but decreased CaMKII levels.
Therefore, the miR-214/CaMKII axis regulates oxidative stress-related injury in CSCs, such as apoptosis, calcium homeostasis disequilibrium, and excessive ROS accumulation.
Collectively, these findings suggest that BMSCs release miR-214-containing exosomes to suppress oxidative stress injury in CSCs through CaMKII silencing.
American Psychological Association (APA)
Wang, Yan& Zhao, Ranzun& Liu, Debin& Deng, Wenwen& Xu, Guanxue& Liu, Weiwei…[et al.]. 2018. Exosomes Derived from miR-214-Enriched Bone Marrow-Derived Mesenchymal Stem Cells Regulate Oxidative Damage in Cardiac Stem Cells by Targeting CaMKII. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-21.
https://search.emarefa.net/detail/BIM-1211541
Modern Language Association (MLA)
Wang, Yan…[et al.]. Exosomes Derived from miR-214-Enriched Bone Marrow-Derived Mesenchymal Stem Cells Regulate Oxidative Damage in Cardiac Stem Cells by Targeting CaMKII. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-21.
https://search.emarefa.net/detail/BIM-1211541
American Medical Association (AMA)
Wang, Yan& Zhao, Ranzun& Liu, Debin& Deng, Wenwen& Xu, Guanxue& Liu, Weiwei…[et al.]. Exosomes Derived from miR-214-Enriched Bone Marrow-Derived Mesenchymal Stem Cells Regulate Oxidative Damage in Cardiac Stem Cells by Targeting CaMKII. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-21.
https://search.emarefa.net/detail/BIM-1211541
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211541