Evaluation of Potential Mechanisms Controlling the Catalase Expression in Breast Cancer Cells
Joint Authors
Buc Calderon, Pedro
Poirel, Hélène A.
Glorieux, Christophe
Sandoval, Juan Marcelo
Dejeans, Nicolas
Nonckreman, Sandrine
Bahloula, Khadija
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Development of cancer cell resistance against prooxidant drugs limits its potential clinical use.
MCF-7 breast cancer cells chronically exposed to ascorbate/menadione became resistant (Resox cells) by increasing mainly catalase activity.
Since catalase appears as an anticancer target, the elucidation of mechanisms regulating its expression is an important issue.
In MCF-7 and Resox cells, karyotype analysis showed that chromosome 11 is not altered compared to healthy mammary epithelial cells.
The genomic gain of catalase locus observed in MCF-7 and Resox cells cannot explain the differential catalase expression.
Since ROS cause DNA lesions, the activation of DNA damage signaling pathways may influence catalase expression.
However, none of the related proteins (i.e., p53, ChK) was activated in Resox cells compared to MCF-7.
The c-abl kinase may lead to catalase protein degradation via posttranslational modifications, but neither ubiquitination nor phosphorylation of catalase was detected after catalase immunoprecipitation.
Catalase mRNA levels did not decrease after actinomycin D treatment in both cell lines.
DNMT inhibitor (5-aza-2′-deoxycytidine) increased catalase protein level in MCF-7 and its resistance to prooxidant drugs.
In line with our previous report, chromatin remodeling appears as the main regulator of catalase expression in breast cancer after chronic exposure to an oxidative stress.
American Psychological Association (APA)
Glorieux, Christophe& Sandoval, Juan Marcelo& Dejeans, Nicolas& Nonckreman, Sandrine& Bahloula, Khadija& Poirel, Hélène A.…[et al.]. 2018. Evaluation of Potential Mechanisms Controlling the Catalase Expression in Breast Cancer Cells. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1211618
Modern Language Association (MLA)
Glorieux, Christophe…[et al.]. Evaluation of Potential Mechanisms Controlling the Catalase Expression in Breast Cancer Cells. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1211618
American Medical Association (AMA)
Glorieux, Christophe& Sandoval, Juan Marcelo& Dejeans, Nicolas& Nonckreman, Sandrine& Bahloula, Khadija& Poirel, Hélène A.…[et al.]. Evaluation of Potential Mechanisms Controlling the Catalase Expression in Breast Cancer Cells. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1211618
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211618