Curcumin Modulates DNA Methyltransferase Functions in a Cellular Model of Diabetic Retinopathy
Joint Authors
Maugeri, Andrea
Basile, Guido
Mazzone, Maria Grazia
Giuliano, Francesco
Barchitta, Martina
Agodi, Antonella
Vinciguerra, Manlio
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-02
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Hyperglycaemia-induced oxidative stress appears to be involved in the aetiology of diabetic retinopathy (DR), a major public health issue, via altering DNA methylation process.
We investigated the effect of hyperglycaemia on retinal DNA methyltransferase (DNMT) expression in diabetic mice, using Gene Expression Omnibus datasets.
We also evaluated the effect of curcumin both on high glucose-induced reactive oxygen species (ROS) production and altered DNMT functions, in a cellular model of DR.
We observed that three months of hyperglycaemia, in insulin-deficient Ins2Akita mice, decrease DNMT1 and DNMT3a expression levels.
In retinal pigment epithelium (RPE) cells, we also demonstrated that high glucose-induced ROS production precedes upregulation of DNMT expression and activity, suggesting that changes in DNMT function could be mediated by oxidative stress via a potential dual effect.
The early effect results in decreased DNMT activity, accompanied by the highest ROS production, while long-term oxidative stress increases DNMT activity and DNMT1 expression.
Interestingly, treatment with 25 μM curcumin for 6 hours restores ROS production, as well as DNMT functions, altered by the exposure of RPE to acute and chronic high glucose concentration.
Our study suggests that curcumin may represent an effective antioxidant compound against DR, via restoring oxidative stress and DNMT functions, though further studies are recommended.
American Psychological Association (APA)
Maugeri, Andrea& Mazzone, Maria Grazia& Giuliano, Francesco& Vinciguerra, Manlio& Basile, Guido& Barchitta, Martina…[et al.]. 2018. Curcumin Modulates DNA Methyltransferase Functions in a Cellular Model of Diabetic Retinopathy. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211626
Modern Language Association (MLA)
Maugeri, Andrea…[et al.]. Curcumin Modulates DNA Methyltransferase Functions in a Cellular Model of Diabetic Retinopathy. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1211626
American Medical Association (AMA)
Maugeri, Andrea& Mazzone, Maria Grazia& Giuliano, Francesco& Vinciguerra, Manlio& Basile, Guido& Barchitta, Martina…[et al.]. Curcumin Modulates DNA Methyltransferase Functions in a Cellular Model of Diabetic Retinopathy. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211626
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211626