The Impact of Moderate Chronic Hypoxia and Hyperoxia on the Level of Apoptotic and Autophagic Proteins in Myocardial Tissue
Joint Authors
Samaja, Michele
Gyongyosi, Alexandra
Terraneo, Laura
Bianciardi, Paola
Tosaki, Arpad
Lekli, Istvan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The redox imbalance and the consequent oxidative stress have been implicated in many pathological conditions, including cardiovascular diseases.
The lack or the excess of O2 supply can alter the redox balance.
The aim of the present study was to understand the heart responses to prolonged hypoxia or hyperoxia and how such situations may activate survival mechanisms or trigger cell death.
Seven-week-old Foxn1 mice were exposed to hypoxia (10% O2), normoxia (21% O2), or hyperoxia (30% O2) for 28 days, then the heart tissue was excised and analyzed.
The alterations in redox balance, housekeeping protein levels, and autophagic and apoptotic process regulation were studied.
The D-ROM test demonstrated an increased oxidative stress in the hypoxic group compared to the hyperoxic group.
The level of hypoxia inducible factor-1 (HIF-1α) was increased by hypoxia while HIF-2α was not affected by treatments.
Chronic hypoxia activated the biochemical markers of autophagy, and we observed elevated levels of Beclin-1 while LC3B-II and p62 were constant.
Nevertheless, we measured significantly enhanced number of TUNEL-positive cells and higher Bax/Bcl2 ratio in hyperoxia with respect to hypoxia.
Surprisingly, our results revealed alterations in the level of housekeeping proteins.
The expression of α-tubulin, total-actin, and GAPDH was increased in the hypoxic group while decreased in the hyperoxic group.
These findings suggest that autophagy is induced in the heart under hypoxia, which may serve as a protective mechanism in response to enhanced oxidative stress.
While prolonged hypoxia-induced autophagy leads to reduced heart apoptosis, low autophagic level in hyperoxia failed to prevent the excessive DNA fragmentation.
American Psychological Association (APA)
Gyongyosi, Alexandra& Terraneo, Laura& Bianciardi, Paola& Tosaki, Arpad& Lekli, Istvan& Samaja, Michele. 2018. The Impact of Moderate Chronic Hypoxia and Hyperoxia on the Level of Apoptotic and Autophagic Proteins in Myocardial Tissue. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211675
Modern Language Association (MLA)
Gyongyosi, Alexandra…[et al.]. The Impact of Moderate Chronic Hypoxia and Hyperoxia on the Level of Apoptotic and Autophagic Proteins in Myocardial Tissue. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1211675
American Medical Association (AMA)
Gyongyosi, Alexandra& Terraneo, Laura& Bianciardi, Paola& Tosaki, Arpad& Lekli, Istvan& Samaja, Michele. The Impact of Moderate Chronic Hypoxia and Hyperoxia on the Level of Apoptotic and Autophagic Proteins in Myocardial Tissue. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211675
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211675