Effects of the Photosystem II Inhibitors CCCP and DCMU on Hydrogen Production by the Unicellular Halotolerant Cyanobacterium Aphanothece halophytica
Joint Authors
Pansook, Sunisa
Incharoensakdi, Aran
Phunpruch, Saranya
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The unicellular halotolerant cyanobacterium Aphanothece halophytica is a potential dark fermentative producer of molecular hydrogen (H2) that produces very little H2 under illumination.
One factor limiting the H2 photoproduction of this cyanobacterium is an inhibition of bidirectional hydrogenase activity by oxygen (O2) obtained from splitting water molecules via photosystem II activity.
The present study aimed to investigate the effects of the photosystem II inhibitors carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) on H2 production of A.
halophytica under light and dark conditions and on photosynthetic and respiratory activities.
The results showed that A.
halophytica treated with CCCP and DCMU produced H2 at three to five times the rate of untreated cells, when exposed to light.
The highest H2 photoproduction rates, 2 .
26 ± 0 .
24 and 3 .
63 ± 0 .
26 μmol H2 g−1 dry weight h−1, were found in cells treated with 0.5 μM CCCP and 50 μM DCMU, respectively.
Without inhibitor treatment, A.
halophytica incubated in the dark showed a significant increase in H2 production compared with cells that were incubated in the light.
Only CCCP treatment increased H2 production of A.
halophytica during dark incubation, because CCCP functions as an uncoupling agent of oxidative phosphorylation.
The highest dark fermentative H2 production rate of 39 .
50 ± 2 .
13 μmol H2 g−1 dry weight h−1 was found in cells treated with 0.5 μM CCCP after 2 h of dark incubation.
Under illumination, CCCP and DCMU inhibited chlorophyll fluorescence, resulting in a low level of O2, which promoted bidirectional hydrogenase activity in A.
halophytica cells.
In addition, only CCCP enhanced the respiration rate, further reducing the O2 level.
In contrast, DCMU reduced the respiration rate in A.
halophytica.
American Psychological Association (APA)
Pansook, Sunisa& Incharoensakdi, Aran& Phunpruch, Saranya. 2019. Effects of the Photosystem II Inhibitors CCCP and DCMU on Hydrogen Production by the Unicellular Halotolerant Cyanobacterium Aphanothece halophytica. The Scientific World Journal،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1211793
Modern Language Association (MLA)
Pansook, Sunisa…[et al.]. Effects of the Photosystem II Inhibitors CCCP and DCMU on Hydrogen Production by the Unicellular Halotolerant Cyanobacterium Aphanothece halophytica. The Scientific World Journal No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1211793
American Medical Association (AMA)
Pansook, Sunisa& Incharoensakdi, Aran& Phunpruch, Saranya. Effects of the Photosystem II Inhibitors CCCP and DCMU on Hydrogen Production by the Unicellular Halotolerant Cyanobacterium Aphanothece halophytica. The Scientific World Journal. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1211793
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211793