Photobiomodulation at Multiple Wavelengths Differentially Modulates Oxidative Stress In Vitro and In Vivo
Joint Authors
Biasotto, M.
Di Lenarda, Roberto
Zacchigna, Serena
Rupel, Katia
Zupin, Luisa
Colliva, Andrea
Kamada, Anselmo
Poropat, Augusto
Ottaviani, Giulia
Gobbo, Margherita
Fanfoni, Lidia
Gratton, Rossella
Santoro, Massimo
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-11
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Photobiomodulation (PBM) is emerging as an effective strategy for the management of multiple inflammatory conditions, including oral mucositis (OM) in cancer patients who receive chemotherapy or radiotherapy.
Still, the poor understanding of the mechanisms by which the light interacts with biological tissues and the heterogeneity of light sources and protocols employed worldwide significantly limits its applicability.
Reactive oxygen species (ROS) are massively generated during the early phases of OM and play a major role in the pathogenesis of inflammation in general.
Here, we report the results of a clinical and experimental study, aimed at evaluating the effect of laser light at different wavelengths on oxidative stress in vivo in oncologic patients suffering from OM and in vitro in two cell types abundantly present within the inflamed oral mucosa, neutrophil polymorphonuclear (PMN) granulocytes, and keratinocytes.
In addition to standard ROS detection methods, we exploited a roGFP2-Orp1 genetically encoded sensor, allowing specific, quantitative, and dynamic imaging of redox events in living cells in response to oxidative stress and PBM.
We found that the various wavelengths differentially modulate ROS production.
In particular, the 660 nm laser light increases ROS production when applied either before or after an oxidative stimulus.
In contrast, the 970 nm laser light exerted a moderate antioxidant activity both in the saliva of OM patients and in both cell types.
The most marked reduction in the levels of ROS was detected in cells exposed either to the 800 nm laser light or to the combination of the three wavelengths.
Overall, our study demonstrates that PBM exerts different effects on the redox state of both PMNs and keratinocytes depending on the used wavelength and prompts the validation of a multiwavelength protocol in the clinical settings.
American Psychological Association (APA)
Rupel, Katia& Zupin, Luisa& Colliva, Andrea& Kamada, Anselmo& Poropat, Augusto& Ottaviani, Giulia…[et al.]. 2018. Photobiomodulation at Multiple Wavelengths Differentially Modulates Oxidative Stress In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1211829
Modern Language Association (MLA)
Rupel, Katia…[et al.]. Photobiomodulation at Multiple Wavelengths Differentially Modulates Oxidative Stress In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1211829
American Medical Association (AMA)
Rupel, Katia& Zupin, Luisa& Colliva, Andrea& Kamada, Anselmo& Poropat, Augusto& Ottaviani, Giulia…[et al.]. Photobiomodulation at Multiple Wavelengths Differentially Modulates Oxidative Stress In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1211829
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211829