Protective Role of UCP2 in Oxidative Stress and Apoptosis during the Silent Phase of an Experimental Model of Epilepsy Induced by Pilocarpine
Joint Authors
Serra, Andrey Jorge
Junior, José Antonio Silva
Dutra, Marina Rascio Henriques
Feliciano, Regiane dos Santos
Jacinto, Kalil Ribeiro
Gouveia, Telma Luciana Furtado
Brigidio, Eduardo
Morris, Mariana
Naffah-Mazzacoratti, Maria da Graça
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-06
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Neuroprotection is a desirable process in many neurological disorders, yet complex mechanisms involved in this field are not completely understood.
The pilocarpine epilepsy model causes potent, seizure-induced excitotoxicity cell death and mitochondria impairment.
The present study is aimed at investigating the role of UCP2, a ROS negative regulator, in the neuroprotection after cholinergic insult.
Our data demonstrated that UCP2 expression was augmented in the rat hippocampus 3 days after status epilepticus (SE), reaching a peak on the fifth day, then returning to basal levels.
Concomitantly, phospho-AKT expression levels were higher in the hippocampus during the early silent phase (5 days after SE).
Additionally, it was demonstrated that the blockade of UCP2 by antisense oligonucleotides (ASO) in SE rats successfully diminished both UCP2 mRNA and protein contents.
SE ASO rats presented increased mitochondrial proapoptotic factor expression, caspase-3 activity, inflammatory cytokine expression, and ROS formation.
Moreover, ASO treatment diminished p-AKT expression and antioxidant enzyme activities after pilocarpine insult.
In conclusion, the present results highlight the neuroprotective actions of UCP2, acting in the inhibition of apoptotic factors and oxidative stress, to increase neuron survival after SE onset.
American Psychological Association (APA)
Dutra, Marina Rascio Henriques& Feliciano, Regiane dos Santos& Jacinto, Kalil Ribeiro& Gouveia, Telma Luciana Furtado& Brigidio, Eduardo& Serra, Andrey Jorge…[et al.]. 2018. Protective Role of UCP2 in Oxidative Stress and Apoptosis during the Silent Phase of an Experimental Model of Epilepsy Induced by Pilocarpine. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211864
Modern Language Association (MLA)
Dutra, Marina Rascio Henriques…[et al.]. Protective Role of UCP2 in Oxidative Stress and Apoptosis during the Silent Phase of an Experimental Model of Epilepsy Induced by Pilocarpine. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1211864
American Medical Association (AMA)
Dutra, Marina Rascio Henriques& Feliciano, Regiane dos Santos& Jacinto, Kalil Ribeiro& Gouveia, Telma Luciana Furtado& Brigidio, Eduardo& Serra, Andrey Jorge…[et al.]. Protective Role of UCP2 in Oxidative Stress and Apoptosis during the Silent Phase of an Experimental Model of Epilepsy Induced by Pilocarpine. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1211864
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211864