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MicroRNA miR-24-3p Reduces Apoptosis and Regulates Keap1-Nrf2 Pathway in Mouse Cardiomyocytes Responding to IschemiaReperfusion Injury
Joint Authors
Xiao, Xu
Lin, Victor
Tran, Kyle
Che, Briana
May, Adam
Lu, Zhigang
Shaw, Daniel H.
Wang, Zhihao
Shaw, Peter X.
Du, Hong-jun
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In recent years, microRNAs (miRNAs) have received increasing attention for their role in ischemia/reperfusion injury (I/RI), and many miRNAs have been demonstrated to play a very important role in cardiac I/RI.
The miRNA miR-24-3p is a tumor suppressor that regulates multiple tumors; however, it remains unclear whether the expression level of miR-24-3p is altered in cardiac cells under I/RI.
In this study, we used mouse primary cardiomyocytes and the H9C2 cardiomyocyte cell line to perform in vitro stimulated ischemia/reperfusion (SI/R) and then detected miR-24-3p expression level using quantitative real-time PCR (qRT-PCR).
We discovered that the expression of miR-24-3p was significantly increased in cardiomyocytes following SI/R, and that the miR-24-3p level was inversely correlated to the ischemia marker HIF-1a.
Furthermore, we transfected cardiomyocytes with miR-24-3p mimic or inhibitor to explore the role of miR-24-3p in cardiomyocyte ischemia/reperfusion injury in vitro.
We performed flow cytometry to detect the apoptotic rate of H9C2 cardiomyocytes and found that the transfection of miR-24-3p mimic resulted in the decrease of the apoptosis rate of cardiomyocytes after SI/R, whereas the transfection of miR-24-3p inhibitor increased the number of apoptotic cardiomyocytes.
These data suggest that the overexpression of miR-24-3p could reduce in vitro myocardial cell apoptosis induced by I/R injury.
Finally, we applied the dual luciferase reporter gene system to verify whether miR-24-3p targets the Keap1 gene, and found that the luciferase signal intensity from a vector carrying the Keap1 wild-type reporter gene was significantly reduced after transfection with miR-24-3p mimic.
The Keap1 protein level was also reduced following the transfection of miR-24-3p.
The results from this study suggest a novel function of miR-24-3p in protecting cardiomyocytes from ischemia/reperfusion injury by the activation of the Nrf2-Keap1 pathway.
American Psychological Association (APA)
Xiao, Xu& Lu, Zhigang& Lin, Victor& May, Adam& Shaw, Daniel H.& Wang, Zhihao…[et al.]. 2018. MicroRNA miR-24-3p Reduces Apoptosis and Regulates Keap1-Nrf2 Pathway in Mouse Cardiomyocytes Responding to IschemiaReperfusion Injury. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1211934
Modern Language Association (MLA)
Xiao, Xu…[et al.]. MicroRNA miR-24-3p Reduces Apoptosis and Regulates Keap1-Nrf2 Pathway in Mouse Cardiomyocytes Responding to IschemiaReperfusion Injury. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1211934
American Medical Association (AMA)
Xiao, Xu& Lu, Zhigang& Lin, Victor& May, Adam& Shaw, Daniel H.& Wang, Zhihao…[et al.]. MicroRNA miR-24-3p Reduces Apoptosis and Regulates Keap1-Nrf2 Pathway in Mouse Cardiomyocytes Responding to IschemiaReperfusion Injury. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1211934
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1211934