Extracorporeal Shock Wave-Supported Adipose-Derived Fresh Stromal Vascular Fraction Preserved Left Ventricular (LV) Function and Inhibited LV Remodeling in Acute Myocardial Infarction in Rat
Joint Authors
Wang, Ching-Jen
Sun, Cheuk-Kwan
Sheu, Jiunn-Jye
Lee, Fan-Yen
Yin, Tsung-Cheng
Sung, Pei-Hsun
Chen, Kuan-Hung
Shao, Pei-Lin
Chen, Yung-Lung
Omran, Mostafa Mohammad
Chung, Sheng-Ying
Lee, Mel S.
Wallace, Christopher Glenn
Yip, Hon-Kan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-22, 22 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-17
Country of Publication
Egypt
No. of Pages
22
Main Subjects
Abstract EN
This study tested the hypothesis that extracorporeal shock wave- (ECSW-) assisted adipose-derived stromal vascular fraction (SVF) therapy could preserve left ventricular ejection fraction (LVEF) and inhibit LV remodeling in a rat after acute myocardial infarction (AMI).
Adult male SD rats were categorized into group 1 (sham control), group 2 (AMI induced by left coronary artery ligation), group 3 [AMI + ECSW (280 impulses at 0.1 mJ/mm2, applied to the chest wall at 3 h, days 3 and 7 after AMI), group 4 [AMI + SVF (1.2 × 106) implanted into the infarct area at 3 h after AMI], and group 5 (AMI + ECSW-SVF).
In vitro, SVF protected H9C2 cells against menadione-induced mitochondrial damage and increased fluorescent intensity of mitochondria in nuclei (p<0.01).
By day 42 after AMI, LVEF was highest in group 1, lowest in group 2, significantly higher in group 5 than in groups 3 and 4, and similar between the latter two groups (all p<0.0001).
LV remodeling and infarcted, fibrotic, and collagen deposition areas as well as apoptotic nuclei exhibited an opposite pattern to LVEF among the groups (all p<0.0001).
Protein expressions of CD31/vWF/eNOS/PGC-1α/α-MHC/mitochondrial cytochrome C exhibited an identical pattern, whilst protein expressions of MMP-9/TNF-α/IL-1β/NF-κB/caspase-3/PARP/Samd3/TGF-β/NOX-1/NOX-2/oxidized protein/β-MHC/BNP exhibited an opposite pattern to LVEF among five groups (all p<0.0001).
Cellular expressions of CXCR4/SDF-1α/Sca-1/c-Kit significantly and progressively increased from groups 1 to 5 (all p<0.0001).
Cellular expression of γ-H2AX/CD68 displayed an opposite pattern to LVEF among the five groups (all p<0.0001).
In conclusion, ECSW-SVF therapy effectively preserved LVEF and inhibited LV remodeling in rat AMI.
American Psychological Association (APA)
Sung, Pei-Hsun& Yin, Tsung-Cheng& Wallace, Christopher Glenn& Chen, Kuan-Hung& Shao, Pei-Lin& Lee, Fan-Yen…[et al.]. 2018. Extracorporeal Shock Wave-Supported Adipose-Derived Fresh Stromal Vascular Fraction Preserved Left Ventricular (LV) Function and Inhibited LV Remodeling in Acute Myocardial Infarction in Rat. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-22.
https://search.emarefa.net/detail/BIM-1212018
Modern Language Association (MLA)
Sung, Pei-Hsun…[et al.]. Extracorporeal Shock Wave-Supported Adipose-Derived Fresh Stromal Vascular Fraction Preserved Left Ventricular (LV) Function and Inhibited LV Remodeling in Acute Myocardial Infarction in Rat. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-22.
https://search.emarefa.net/detail/BIM-1212018
American Medical Association (AMA)
Sung, Pei-Hsun& Yin, Tsung-Cheng& Wallace, Christopher Glenn& Chen, Kuan-Hung& Shao, Pei-Lin& Lee, Fan-Yen…[et al.]. Extracorporeal Shock Wave-Supported Adipose-Derived Fresh Stromal Vascular Fraction Preserved Left Ventricular (LV) Function and Inhibited LV Remodeling in Acute Myocardial Infarction in Rat. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-22.
https://search.emarefa.net/detail/BIM-1212018
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212018