L-Arginine Enhances Protein Synthesis by Phosphorylating mTOR (Thr 2446) in a Nitric Oxide-Dependent Manner in C2C12 Cells
Joint Authors
Lin, Hai
Wang, Ruxia
Wang, Xiaojuan
Zhao, Jingpeng
Jiao, Hongchao
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-26
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Muscle atrophy may arise from many factors such as inactivity, malnutrition, and inflammation.
In the present study, we investigated the stimulatory effect of nitric oxide (NO) on muscle protein synthesis.
Primarily, C2C12 cells were supplied with extra L-arginine (L-Arg) in the culture media.
L-Arg supplementation increased the activity of inducible nitric oxide synthase (iNOS), the rate of protein synthesis, and the phosphorylation of mTOR (Thr 2446) and p70S6K (Thr 389).
L-NAME, an NOS inhibitor, decreased NO concentrations within cells and abolished the stimulatory effect of L-Arg on protein synthesis and the phosphorylation of mTOR and p70S6K.
In contrast, SNP (sodium nitroprusside), an NO donor, increased NO concentrations, enhanced protein synthesis, and upregulated mTOR and p70S6K phosphorylation, regardless of L-NAME treatment.
Blocking mTOR with rapamycin abolished the stimulatory effect of both L-Arg and SNP on protein synthesis and p70S6K phosphorylation.
These results indicate that L-Arg stimulates protein synthesis via the activation of the mTOR (Thr 2446)/p70S6K signaling pathway in an NO-dependent manner.
American Psychological Association (APA)
Wang, Ruxia& Jiao, Hongchao& Zhao, Jingpeng& Wang, Xiaojuan& Lin, Hai. 2018. L-Arginine Enhances Protein Synthesis by Phosphorylating mTOR (Thr 2446) in a Nitric Oxide-Dependent Manner in C2C12 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1212036
Modern Language Association (MLA)
Wang, Ruxia…[et al.]. L-Arginine Enhances Protein Synthesis by Phosphorylating mTOR (Thr 2446) in a Nitric Oxide-Dependent Manner in C2C12 Cells. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1212036
American Medical Association (AMA)
Wang, Ruxia& Jiao, Hongchao& Zhao, Jingpeng& Wang, Xiaojuan& Lin, Hai. L-Arginine Enhances Protein Synthesis by Phosphorylating mTOR (Thr 2446) in a Nitric Oxide-Dependent Manner in C2C12 Cells. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1212036
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212036