Biotoxicological Analyses of Trimeroside from Baccharis trimera Using a Battery of In Vitro Test Systems
Joint Authors
Silva, Juliana da
dos Santos, Marcela S.
Ferraz, Alexandre B. F.
Picada, Jaqueline N.
Grivicich, Ivana
Menezes, Ana Paula Simões
de Barros, Francisco Maikon Corrêa
Lemes, Maria Luisa Brodt
Rossatto, Raíssa R.
Feistel, Cleverson
de Almeida, Indara Dedigo
Prado, Lismare
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The use in folk medicine of Baccharis trimera and recent studies on DNA damage by oxidative stress mechanisms have motivated this study.
We investigated the biotoxicological effects of trimeroside from this plant.
Aqueous extract from aerial parts of B.
trimera was fractioned by flash chromatography for further isolation by thin-layer chromatography.
The novel nor-monoterpene glycoside, trimeroside, and three flavonoids, cirsimaritin, luteolin and quercetin, were isolated.
The genotoxic and mutagenic potential of trimeroside was determined by Salmonella/microsome (TA98 and TA100), comet assay, and cytokinesis-block micronucleus cytome assay (CBMN-cyt) in HepG2 cells.
We also screened trimeroside into different human tumoral cell lines by sulforhodamine B (SRB) assay.
Mutagenicity was detected in TA100 strain with metabolic activation.
Genotoxic effects were not observed in HepG2 by comet assay.
However, a decrease in the nuclear index division in the 2.0 mg·mL−1 concentration and an increase of nucleoplasmic bridges in the 1.5 mg·mL−1 concentration were detected by CBMN-cyt assay indicating cytotoxic and mutagenic effects.
In SRB assay, trimeroside showed weak antiproliferative activity against the cell lines.
American Psychological Association (APA)
dos Santos, Marcela S.& Silva, Juliana da& Menezes, Ana Paula Simões& de Barros, Francisco Maikon Corrêa& Lemes, Maria Luisa Brodt& Rossatto, Raíssa R.…[et al.]. 2018. Biotoxicological Analyses of Trimeroside from Baccharis trimera Using a Battery of In Vitro Test Systems. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1212080
Modern Language Association (MLA)
dos Santos, Marcela S.…[et al.]. Biotoxicological Analyses of Trimeroside from Baccharis trimera Using a Battery of In Vitro Test Systems. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1212080
American Medical Association (AMA)
dos Santos, Marcela S.& Silva, Juliana da& Menezes, Ana Paula Simões& de Barros, Francisco Maikon Corrêa& Lemes, Maria Luisa Brodt& Rossatto, Raíssa R.…[et al.]. Biotoxicological Analyses of Trimeroside from Baccharis trimera Using a Battery of In Vitro Test Systems. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1212080
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212080