Atorvastatin Downregulates In Vitro Methyl Methanesulfonate and Cyclophosphamide Alkylation-Mediated Cellular and DNA Injuries
Joint Authors
Alessandra Fortes Aiub, Claudia
Felzenszwalb, Israel
Justo, Graça
Araujo-Lima, Carlos F.
Christoni, Larissa S. A.
Soeiro, Maria N. C.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Statins are 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors, and this class of drugs has been studied as protective agents against DNA damages.
Alkylating agents (AAs) are able to induce alkylation in macromolecules, causing DNA damage, as DNA methylation.
Our objective was to evaluate atorvastatin (AVA) antimutagenic, cytoprotective, and antigenotoxic potentials against DNA lesions caused by AA.
AVA chemopreventive ability was evaluated using antimutagenicity assays (Salmonella/microsome assay), cytotoxicity, cell cycle, and genotoxicity assays in HepG2 cells.
The cells were cotreated with AVA and the AA methyl methanesulfonate (MMS) or cyclophosphamide (CPA).
Our datum showed that AVA reduces the alkylation-mediated DNA damage in different in vitro experimental models.
Cytoprotection of AVA at low doses (0.1–1.0 μM) was observed after 24 h of cotreatment with MMS or CPA at their LC50, causing an increase in HepG2 survival rates.
After all, AVA at 10 μM and 25 μM had decreased effect in micronucleus formation in HepG2 cells and restored cell cycle alterations induced by MMS and CPA.
This study supports the hypothesis that statins can be chemopreventive agents, acting as antimutagenic, antigenotoxic, and cytoprotective components, specifically against alkylating agents of DNA.
American Psychological Association (APA)
Araujo-Lima, Carlos F.& Christoni, Larissa S. A.& Justo, Graça& Soeiro, Maria N. C.& Alessandra Fortes Aiub, Claudia& Felzenszwalb, Israel. 2018. Atorvastatin Downregulates In Vitro Methyl Methanesulfonate and Cyclophosphamide Alkylation-Mediated Cellular and DNA Injuries. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1212085
Modern Language Association (MLA)
Araujo-Lima, Carlos F.…[et al.]. Atorvastatin Downregulates In Vitro Methyl Methanesulfonate and Cyclophosphamide Alkylation-Mediated Cellular and DNA Injuries. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1212085
American Medical Association (AMA)
Araujo-Lima, Carlos F.& Christoni, Larissa S. A.& Justo, Graça& Soeiro, Maria N. C.& Alessandra Fortes Aiub, Claudia& Felzenszwalb, Israel. Atorvastatin Downregulates In Vitro Methyl Methanesulfonate and Cyclophosphamide Alkylation-Mediated Cellular and DNA Injuries. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1212085
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212085