Cafestol Inhibits Cyclic-Strain-Induced Interleukin-8, Intercellular Adhesion Molecule-1, and Monocyte Chemoattractant Protein-1 Production in Vascular Endothelial Cells
Joint Authors
Chao, Hung-Hsing
Liu, Ju-Chi
Chen, Jin-Jer
Hao, Wen-Rui
Sung, Li-Chin
Chen, Chun-Chao
Chen, Po-Yuan
Cheng, Tzu-Hurng
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Moderate coffee consumption is inversely associated with cardiovascular disease mortality; however, mechanisms underlying this causal effect remain unclear.
Cafestol, a diterpene found in coffee, has various properties, including an anti-inflammatory property.
This study investigated the effect of cafestol on cyclic-strain-induced inflammatory molecule secretion in vascular endothelial cells.
Cells were cultured under static or cyclic strain conditions, and the secretion of inflammatory molecules was determined using enzyme-linked immunosorbent assay.
The effects of cafestol on mitogen-activated protein kinases (MAPK), heme oxygenase-1 (HO-1), and sirtuin 1 (Sirt1) signaling pathways were examined using Western blotting and specific inhibitors.
Cafestol attenuated cyclic-strain-stimulated intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein- (MCP-) 1, and interleukin- (IL-) 8 secretion.
Cafestol inhibited the cyclic-strain-induced phosphorylation of extracellular signal-regulated kinase and p38 MAPK.
By contrast, cafestol upregulated cyclic-strain-induced HO-1 and Sirt1 expression.
The addition of zinc protoporphyrin IX, sirtinol, or Sirt1 silencing (transfected with Sirt1 siRNA) significantly attenuated cafestol-mediated modulatory effects on cyclic-strain-stimulated ICAM-1, MCP-1, and IL-8 secretion.
This is the first study to report that cafestol inhibited cyclic-strain-induced inflammatory molecule secretion, possibly through the activation of HO-1 and Sirt1 in endothelial cells.
The results provide valuable insights into molecular pathways that may contribute to the effects of cafestol.
American Psychological Association (APA)
Hao, Wen-Rui& Sung, Li-Chin& Chen, Chun-Chao& Chen, Po-Yuan& Cheng, Tzu-Hurng& Chao, Hung-Hsing…[et al.]. 2018. Cafestol Inhibits Cyclic-Strain-Induced Interleukin-8, Intercellular Adhesion Molecule-1, and Monocyte Chemoattractant Protein-1 Production in Vascular Endothelial Cells. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1212107
Modern Language Association (MLA)
Hao, Wen-Rui…[et al.]. Cafestol Inhibits Cyclic-Strain-Induced Interleukin-8, Intercellular Adhesion Molecule-1, and Monocyte Chemoattractant Protein-1 Production in Vascular Endothelial Cells. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1212107
American Medical Association (AMA)
Hao, Wen-Rui& Sung, Li-Chin& Chen, Chun-Chao& Chen, Po-Yuan& Cheng, Tzu-Hurng& Chao, Hung-Hsing…[et al.]. Cafestol Inhibits Cyclic-Strain-Induced Interleukin-8, Intercellular Adhesion Molecule-1, and Monocyte Chemoattractant Protein-1 Production in Vascular Endothelial Cells. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1212107
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212107