Urinary Mitochondrial DNA Identifies Renal Dysfunction and Mitochondrial Damage in Sepsis-Induced Acute Kidney Injury
Joint Authors
Liu, Song
Wu, Xiuwen
Gu, Guosheng
Ren, Jianan
Hu, Qiongyuan
Wu, Jie
Guo, Kun
Li, Jieshou
Ren, Huajian
Wang, Gefei
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-26
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Background.
Recent animal studies have shown that mitochondrial dysfunction initiates and accelerates renal injury in sepsis, but its role in sepsis remains unknown.
Mitochondrial stress or dying cells can lead to fragmentation of the mitochondrial genome, which is considered a surrogate marker of mitochondrial dysfunction.
Therefore, we evaluated the efficiency of urinary mitochondrial DNA (UmtDNA) as a marker of renal dysfunction during sepsis-induced acute kidney injury (AKI).
Methods.
We isolated DNA from plasma and urine of patients.
mtDNA levels were quantified by quantitative PCR.
Sepsis patients were divided into no AKI, mild AKI, and severe AKI groups according to RIFLE criteria.
Additionally, cecal ligation and puncture (CLP) was established in rats to evaluate the association between UmtDNA and mitochondrial function.
Results.
A total of 52 (49.5%) developed AKI among enrolled sepsis patients.
Increased systemic mtDNA did not correlate with systemic inflammation or acute renal dysfunction in sepsis patients, while AKI did not have an additional effect on circulating mtDNA levels.
In contrast, UmtDNA was significantly enriched in severe AKI patients compared with that in the mild AKI or no AKI group, positively correlated with plasma creatinine, urinary neutrophil gelatinase-associated lipocalin, and kidney injury molecule-1, and inversely with the estimated glomerular filtration rate.
Additionally, UmtDNA increased in rats following CLP-induced sepsis.
UmtDNA was predictive of AKI development and correlated with plasma creatinine and blood urea nitrogen in the rat sepsis model.
Finally, the UmtDNA level was inversely correlated with the cortical mtDNA copy number and relative expression of mitochondrial gene in the kidney.
Conclusion.
An elevated UmtDNA level correlates with mitochondrial dysfunction and renal injury in sepsis patients, indicating renal mitochondrial injury induced by sepsis.
Therefore, UmtDNA may be regarded as a valuable biomarker for the occurrence of AKI and the development of mitochondria-targeted therapies following sepsis-induced AKI.
American Psychological Association (APA)
Hu, Qiongyuan& Ren, Jianan& Ren, Huajian& Wu, Jie& Wu, Xiuwen& Liu, Song…[et al.]. 2018. Urinary Mitochondrial DNA Identifies Renal Dysfunction and Mitochondrial Damage in Sepsis-Induced Acute Kidney Injury. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1212149
Modern Language Association (MLA)
Hu, Qiongyuan…[et al.]. Urinary Mitochondrial DNA Identifies Renal Dysfunction and Mitochondrial Damage in Sepsis-Induced Acute Kidney Injury. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1212149
American Medical Association (AMA)
Hu, Qiongyuan& Ren, Jianan& Ren, Huajian& Wu, Jie& Wu, Xiuwen& Liu, Song…[et al.]. Urinary Mitochondrial DNA Identifies Renal Dysfunction and Mitochondrial Damage in Sepsis-Induced Acute Kidney Injury. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1212149
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212149