Inhibition of Protein Aggregation by Several Antioxidants
Joint Authors
Tušek Žnidarič, Magda
Žerovnik, Eva
Hasanbašić, Samra
Jahić, Alma
Berbić, Selma
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-25
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Amyloid fibril formation is a shared property of all proteins; therefore, model proteins can be used to study this process.
We measured protein aggregation of the model amyloid-forming protein stefin B in the presence and absence of several antioxidants.
Amyloid fibril formation by stefin B was routinely induced at pH 5 and 10% TFE, at room temperature.
The effects of antioxidants NAC, vitamin C, vitamin E, and the three polyphenols resveratrol, quercetin, and curcumin on the kinetics of fibril formation were followed using ThT fluorescence.
Concomitantly, the morphology and amount of the aggregates and fibrils were checked by transmission electron microscopy (TEM).
The concentration of the antioxidants was varied, and it was observed that different modes of action apply at low or high concentrations relative to the binding constant.
In order to obtain more insight into the possible mode of binding, docking of NAC, vitamin C, and all three polyphenols was done to the monomeric form of stefin B.
American Psychological Association (APA)
Hasanbašić, Samra& Jahić, Alma& Berbić, Selma& Tušek Žnidarič, Magda& Žerovnik, Eva. 2018. Inhibition of Protein Aggregation by Several Antioxidants. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1212287
Modern Language Association (MLA)
Hasanbašić, Samra…[et al.]. Inhibition of Protein Aggregation by Several Antioxidants. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1212287
American Medical Association (AMA)
Hasanbašić, Samra& Jahić, Alma& Berbić, Selma& Tušek Žnidarič, Magda& Žerovnik, Eva. Inhibition of Protein Aggregation by Several Antioxidants. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1212287
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212287