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Protective Effect of Melatonin against Oxidative Stress-Induced Apoptosis and Enhanced Autophagy in Human Retinal Pigment Epithelium Cells
Joint Authors
Chang, Chih-Chao
Huang, Tien-Yi
Chen, Hsin-Yuan
Huang, Tsui-Chin
Lin, Li-Chun
Chang, Yen-Jui
Hsia, Shih-Min
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-05
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Age-related macular degeneration (AMD) affects the retinal macula and results in loss of vision, and AMD is the primary cause of blindness and severe visual impairment among elderly people worldwide.
AMD is characterized by the accumulation of drusen in the Bruch’s membrane and dysfunction of retinal pigment epithelial (RPE) cells and photoreceptors.
The pathogenesis of AMD remains unclear, and no effective treatment exists.
Accumulating evidence indicates that oxidative stress plays a critical role in RPE cell degeneration and AMD.
Melatonin is an antioxidant that scavenges free radicals, and it has anti-inflammatory, antitumor, and antiangiogenic effects.
This study investigated the antioxidative, antiapoptotic, and autophagic effects of melatonin on oxidative damage to RPE cells.
We used hydrogen peroxide (H2O2) to stimulate reactive oxygen species production to cause cell apoptosis in ARPE-19 cell lines.
Our findings revealed that treatment with melatonin significantly inhibited H2O2-induced RPE cell damage, decreased the apoptotic rate, increased the mitochondrial membrane potential, and increased the autophagy effect.
Furthermore, melatonin reduced the Bax/Bcl-2 ratio and the expression levels of the apoptosis-associated proteins cytochrome c and caspase 7.
Additionally, melatonin upregulated the expression of the autophagy-related proteins LC3-II and Beclin-1 and downregulated the expression of p62.
Thus, melatonin’s effects on autophagy and apoptosis can protect against H2O2-induced oxidative damage in human RPE cells.
Melatonin may have multiple protective effects on human RPE cells against H2O2-induced oxidative damage.
American Psychological Association (APA)
Chang, Chih-Chao& Huang, Tien-Yi& Chen, Hsin-Yuan& Huang, Tsui-Chin& Lin, Li-Chun& Chang, Yen-Jui…[et al.]. 2018. Protective Effect of Melatonin against Oxidative Stress-Induced Apoptosis and Enhanced Autophagy in Human Retinal Pigment Epithelium Cells. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1212332
Modern Language Association (MLA)
Chang, Chih-Chao…[et al.]. Protective Effect of Melatonin against Oxidative Stress-Induced Apoptosis and Enhanced Autophagy in Human Retinal Pigment Epithelium Cells. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1212332
American Medical Association (AMA)
Chang, Chih-Chao& Huang, Tien-Yi& Chen, Hsin-Yuan& Huang, Tsui-Chin& Lin, Li-Chun& Chang, Yen-Jui…[et al.]. Protective Effect of Melatonin against Oxidative Stress-Induced Apoptosis and Enhanced Autophagy in Human Retinal Pigment Epithelium Cells. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1212332
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212332